Modification of radiation and dihydroxyanthraquinone-induced cell lethality by cysteamine and n-ethylmaleimide

Conference · Sat Sep 01 04:00:00 EDT 1984 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

OSTI ID:6110434

Kimler, B F; Cheng, C C

The effect of alteration of sulfhydryl levels on the cell lethality induced by ionizing radiation and dihydroxyanthraquinone (DHAQ) was investigated in cultured Chinese hamster V79 cells. DHAQ produces a potentiation of radiation-induced cell lethality, both by increasing the slope and decreasing the shoulder of the survival curve. It has been suggested that DHAQ functions through the production of free radicals which then produce DNA strand breaks and crosslinks, resulting in cytotoxicity. If this mode of action predominates, then one would expect to be able to change the degree of cell kill by modifying conditions such that free radical processes were altered. This was accomplished by the addition of N-ethylmaleimide (NEM) or Cysteamine (CYS) to the culture medium during treatment with DHAQ. It was observed that the combination of DHAQ and NEM did not produce more cytotoxicity than would be expected from an additive interaction. Likewise, CYS did not reduce the cytotoxicity induced by DHAQ. When cells were treated with DHAQ and radiation plus either NEM or CYS, the resultant survival was consistent with an additive interaction between the potentiation of DHAQ for radiation-induced cell kill and the extra effect of NEM or CYS.

• Research Organization: Univ. of Kansas Medical Center, Kansas City
• OSTI ID: 6110434
• Report Number(s): CONF-8311204-
• Conference Information: Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Journal Volume: 10:9
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL ISOTOPES
AMINES
ANIMAL CELLS
ANIMALS
ANTHRAQUINONES
ANTIMITOTIC DRUGS
AROMATICS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL KILLING
CESIUM 137
CESIUM ISOTOPES
CONNECTIVE TISSUE CELLS
DATA
DNA
DRUGS
DYES
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
FIBROBLASTS
GAMMA RADIATION
HAMSTERS
HYDROXY COMPOUNDS
IMIDES
INFORMATION
IONIZING RADIATIONS
ISOTOPES
MAMMALS
MEA
NEM
NUCLEI
NUCLEIC ACIDS
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
QUINIZARIN
QUINONES
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
RADIOSENSITIVITY EFFECTS
RADIOSENSITIZERS
RODENTS
SOMATIC CELLS
STRAND BREAKS
THIOLS
VERTEBRATES
YEARS LIVING RADIOISOTOPES