Effect of dihydroxyanthraquinone (DHAQ) and radiation on the survival of cultured Chinese hamster ovary cells

Journal Article · Tue Feb 01 04:00:00 EST 1983 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575996· OSTI ID:5682809

Kimler, B F

Dihydroxyanthraquinone (DHAQ) is currently being tested as a cancer chemotherapeutic agent because of its structural similarity to Adriamycin (ADR) and other DNA-intercalating antibiotics. The interaction of DHAQ and ionizing radiation on the induction of cell lethality was investigated in Chinese hamster ovary cells in culture. In asynchronous populations of cells, DHAQ produced a slight enhancement of radiation-induced cell lethality as evidenced by changes in both shoulder and slope of the radiation dose-survival curves. However, DHAQ had no effect on either the extent or time course of recovery from sublethal radiation damage. In synchronous populations of cells treated at various times before or after selection in mitosis, the combination of DHAQ and radiation produced greater cell killing than that predicted based on simple additivity of effect, with a decided enhancement for cells treated during S phase. These results indicate that DHAQ is similar to other DNA-intercalating antibiotics in regard to the interaction with ionizing radiation to produce cell lethality.

• Research Organization: Univ. of Kansas Medical Center, Kansas City
• OSTI ID: 5682809
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 93:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTHRAQUINONES
AROMATICS
CELL CULTURES
CELL KILLING
CONNECTIVE TISSUE CELLS
DYES
ELECTROMAGNETIC RADIATION
FIBROBLASTS
HAMSTERS
HYDROXY COMPOUNDS
IONIZING RADIATIONS
MAMMALS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
QUINIZARIN
QUINONES
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
RODENTS
SOMATIC CELLS
SURVIVAL CURVES
SYNCHRONOUS CULTURES
VERTEBRATES
X RADIATION