Repair-defective mutants of Alteromonas espejiana, the host for bacteriophage PM2

Journal Article · Wed Feb 01 04:00:00 EST 1984 · J. Bacteriol.; (United States)

OSTI ID:6110358

Zerler, B R; Wallace, S S

The in vivo repair processes of Alteromonas espejiana, the host for bacteriophage PM2, were characterized, and UV- and methyl methanesulfonate (MMS)-sensitive mutants were isolated. Wild-type A. espejiana cells were capable of photoreactivation, excision, recombination, and inducible repair. There was no detecttable pyrimidine dimer-DNA N-glycosylase activity, and pyrimidine dimer removal appeared to occur by a pathway analogous to the Escherichia coli Uvr pathway. The UV- and MMS-sensitive mutants of A. espejiana included three groups, each containing at least one mutation involved with excision, recombination, or inducible repair. One group that was UV sensitive but not sensitive to MMS or X rays showed a decreased ability to excise pyrimidine dimers. Mutants in this group were also sensitive to psoralen plus near-UV light and were phenotypically analogous to the E. coli uvr mutants. A second group was UV and MMS sensitive but not sensitive to X rays and appeared to contain mutations in a gene(s) involved in recombination repair. These recombination-deficient mutants differed from the E. coli rec mutants, which are MMS and X-ray sensitive. The third group of A. espejiana mutants was sensitive to UV, MMS, and X rays. These mutants were recombination deficient, lacked inducible repair, and were phenotypically similar to E. coli recA mutants.

• Research Organization: New York Medical Coll., Valhalla
• OSTI ID: 6110358
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 157:2; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
560302 -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BACTERIOPHAGES
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DATA
DNA REPAIR
ELECTROMAGNETIC RADIATION
ESTERS
EXPERIMENTAL DATA
INFORMATION
IONIZING RADIATIONS
METHYL METHANESULFONATE
MICROORGANISMS
MUTAGENS
MUTANTS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARASITES
PYRIMIDINE DIMERS
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
SENSITIVITY
SULFONIC ACID ESTERS
ULTRAVIOLET RADIATION
VIRUSES
X RADIATION