Separations of chemically different particles by capillary electrophoresis

Journal Article · Thu Nov 15 04:00:00 EST 1990 · Analytical Chemistry (Washington); (USA)

DOI:https://doi.org/10.1021/ac00221a014· OSTI ID:6108461

Jones, H K; Ballou, N E ^[1]

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1. Pacific Northwest Laboratory, Richland, WA (USA)

Fast, well-resolved separations of mixtures of chemically different latex particles (different numbers of attached carboxylate or sulfate groups) have been successfully achieved by capillary electrophoresis. Best experimental conditions of column inside diameter and length, buffer concentration and pH, and applied voltage were identified for the separations. Electrophoretic migration times and mobilities were determined. The separations were characterized as to numbers of theoretical plates, selectivities, and resolution at different pH and applied voltage values. Numbers of theoretical plates ranged from about 400 to 3,000. Selectivities ranged from about 0.1 to 0.4, and resolution values ranged from 0.8 to 2.3. Electrophoretic mobilities varied especially at the higher applied voltages.

• OSTI ID: 6108461
• Journal Information: Analytical Chemistry (Washington); (USA), Journal Name: Analytical Chemistry (Washington); (USA) Vol. 62:22; ISSN 0003-2700; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400105* -- Separation Procedures
CALCULATION METHODS
DATA
ELASTOMERS
ELECTROPHORESIS
EXPERIMENTAL DATA
EXTRACTION APPARATUSES
EXTRACTION COLUMNS
INFORMATION
LATEX
MEASURING METHODS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC POLYMERS
POLYMERS
RUBBERS
SEPARATION EQUIPMENT
THEORETICAL DATA