Temperature programming in capillary zone electrophoresis
- Iowa State Univ., Ames, IA (United States)
A now type of selectivity in capillary zone electrophoresis (CZE) based on temperature programming is presented. By using a buffer system with a large temperature coefficient, e.g. Tris buffer, the pH within the capillary can be adjusted in situ by simply controlling the temperature of the capillary. The electromigration behavior of weak acids is found to be influenced by both temperature-induced pH changes and viscosity changes. The effect of pH on the electrophoretic mobilities of analytes is most significant if the range of pH change matches the acidity constants, pK{sub a}, of the analytes. The implementation of temperature programming in both temporal and positional modes in CZE are illustrated by the separation of a mixture of fluorescent organic acids. 29 refs., 4 figs., 2 tabs.
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- W-7405-ENG-82
- OSTI ID:
- 589058
- Journal Information:
- Analytical Chemistry (Washington), Journal Name: Analytical Chemistry (Washington) Journal Issue: 5 Vol. 64; ISSN ANCHAM; ISSN 0003-2700
- Country of Publication:
- United States
- Language:
- English
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