Autoradiographic localization of thyrotropin releasing hormone (TRH) receptors in the central nervous system
Thesis/Dissertation
·
OSTI ID:6105228
Quantitative autoradiography was used to examine the distribution of thyrotropin-releasing hormone (TRH) receptors in the rat and human central nervous system (CNS). The binding of (/sup 3/H)-3-methyl-histidine/sup 2/-TRH ((/sup 3/H)-MeTRH) to TRH receptors was saturable, of a high affinity (K/sub d/ = 5 nM), and specific for TRH analogs. Studies with neurotoxins ibotenic acid and 6-hydroxydopamine (6-OHDA) suggest that TRH receptors within the amygdala are predominantly located on cell bodies, and not nerve terminals. Finally, an examination was made of the concentrations of TRH receptors in spinal cords of patients with amyotrophic lateral sclerosis (ALS), a degenerative disease of the motor neurons located in Lamina IX. Large decreases in TRH receptors were noted in ALS spinal cords, when compared to non-neurological controls, probably reflecting the loss of motor neurons. In addition, decreases in the TRH receptor concentration of Lamina II were observed. This finding may reflect the sensitivity of neurons throughout the CNS to the pathophysiologic mechanisms of neuronal degeneration which cause ALS.
- Research Organization:
- Pennsylvania Univ., Philadelphia (USA)
- OSTI ID:
- 6105228
- Country of Publication:
- United States
- Language:
- English
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Quantitative autoradiography of TRH receptors and radioimmunoassay of TRH in the cat central nervous system
Journal Article
·
Sun Feb 10 23:00:00 EST 1985
· Life Sci.; (United States)
·
OSTI ID:5416295
Thyrotropin-releasing hormone (TRH) receptors. Localization by light microscopic autoradiography in rat brain using (/sup 3/H)(3-Me-His/sub 2/)TRH as the radioligand
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Thu Jan 31 23:00:00 EST 1985
· J. Neurosci.; (United States)
·
OSTI ID:5480744
Quantitative autoradiography of TRH receptors and radioimmunoassay of TRH in the cat central nervous system
Conference
·
Tue Mar 04 23:00:00 EST 1986
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:6936465
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
CENTRAL NERVOUS SYSTEM
DATA
DISEASES
EXPERIMENTAL DATA
HORMONES
INFORMATION
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
MAN
MEMBRANE PROTEINS
NERVOUS SYSTEM
NERVOUS SYSTEM DISEASES
NUMERICAL DATA
ORGANIC COMPOUNDS
PATHOLOGY
PATIENTS
PEPTIDE HORMONES
PITUITARY HORMONES
PRIMATES
PROTEINS
RATS
REACTION KINETICS
RECEPTORS
RODENTS
TRACER TECHNIQUES
VERTEBRATES
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
CENTRAL NERVOUS SYSTEM
DATA
DISEASES
EXPERIMENTAL DATA
HORMONES
INFORMATION
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
MAN
MEMBRANE PROTEINS
NERVOUS SYSTEM
NERVOUS SYSTEM DISEASES
NUMERICAL DATA
ORGANIC COMPOUNDS
PATHOLOGY
PATIENTS
PEPTIDE HORMONES
PITUITARY HORMONES
PRIMATES
PROTEINS
RATS
REACTION KINETICS
RECEPTORS
RODENTS
TRACER TECHNIQUES
VERTEBRATES