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Thyrotropin-releasing hormone (TRH) receptors. Localization by light microscopic autoradiography in rat brain using (/sup 3/H)(3-Me-His/sub 2/)TRH as the radioligand

Journal Article · · J. Neurosci.; (United States)
OSTI ID:5480744
;
Thyrotropin releasing hormone (TRH) is a putative neurotransmitter in both the central and peripheral nervous system. In the present report, we have used autoradiography coupled with densitometric analysis of tritium-sensitive film to investigate the distribution of (/sup 3/H)(3-Me-His2)TRH ((/sup 3/H)MeTRH)-binding sizes in the rat brain. Previous pharmacological reports have established that many of these (/sup 3/H)MeTRH-binding sites have a structure-activity profile consistent with being a physiological TRH receptor. A high level of TRH receptors were observed in the accessory olfactory bulb, lateral nucleus of the amygdala, dentate gyrus, and entorhinal cortex. Moderate levels of TRH receptors were observed in the rhinal cortex, hypothalamus, superior colliculus, several brainstem motor nuclei, and lamina I of the spinal trigeminal nucleus pars candalis, while low concentrations of receptors are present in the cerebral cortex, striatum and ventral horn of the spinal cord. Very low levels of receptors were observed in the globus pallidus and in most nuclei of the dorsal thalamus. Comparisons of the distribution of TRH receptors to TRH-immunoreactive content indicates that, while in some areas of the brain there is a rough correlation between levels of TRH peptide and its receptor, in most brain areas there is little obvious correlation between the two. While such a discrepancy has been observed for other peptides and their receptors, the extensive distribution of TRH receptors in the central nervous system does provide an explanation for the variety of behavioral effects observed when TRH is infused into the central nervous system.
Research Organization:
Medical Research Council Neurochemical Pharmacology Unit, Cambridge, England
OSTI ID:
5480744
Journal Information:
J. Neurosci.; (United States), Journal Name: J. Neurosci.; (United States) Vol. 5:2; ISSN JNRSD
Country of Publication:
United States
Language:
English

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Related Subjects

550801* -- Morphology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTORADIOGRAPHY
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CEREBRAL CORTEX
CEREBRUM
DISTRIBUTION
HORMONES
HYPOTHALAMUS
LABELLED COMPOUNDS
MAMMALS
MICROSCOPY
NERVOUS SYSTEM
OLFACTORY BULBS
ORGANS
PEPTIDE HORMONES
RATS
RECEPTORS
RODENTS
THALAMUS
TISSUE DISTRIBUTION
TRH
TRITIUM COMPOUNDS
VERTEBRATES