Glucose tracer, kinetics and turnover in monkeys and chickens infused with ethanol, 1,3-butanediol, or fructose
Mixtures of (2-/sup 3/H) and (U-/sup 14/C) glucose were injected as single doses into fasted cynomolgus monkeys to assess glucose tracer kinetics and obtain rates of turnover. Data were treated by stochastic and compartmental analyses and results from both analyses closely agreed. However, (2-/sup 3/H) data analyzed by the compartmental analysis required three pools to fit the glucose disappearance curve while (6-/sup 3/H) data fit a two or three pool model equally well. Turnover rates averaged 4.9-4.0, and 3.0 mg/min x kg/sup -1/ body weight with (2-/sup 3/H), 6-/sup 3/H) and (U-/sup 14/C) glucose tracers, respectively. The data heuristically suggest that the slow turnover pool that was necessary to fit (2-/sup 3/H) glucose data is related to isotope discrimination. The effects of four treatment solutions on (6-/sup 3/H) glucose metabolism in monkeys were examined. The solutions and their rates of infusion (umoles/min x kg/sup -1/) were: (1) ethanol, 110; (2) 1,3-butanediol, 110; (3) fructose, 30; and (4) ethanol pus fructose, 110 and 30, respectively. The glucose clearance rate was lowest during the ethanol plus fructose infusions. Ethanol infusions (222 or 444 umoles/min x kg/sup -1/ body weight) in chickens (1500 g) fasted 64 hours did not cause hypoglycemia although the high dose slightly decreased the rate of glucose turnover 15% (14.0 versus 12.0 mg/min x kg/sup -1/). It was further found that neither the hepatic cytosolic nor the mitochondrial redox state significantly changed in chickens infused with the high dose of ethanol. The unchanged hepatic metabolite ratios in chickens are consistent with their unusual resistance to ethanol-induced hypoglycemia.
- Research Organization:
- Michigan State Univ., East Lansing (USA)
- OSTI ID:
- 6104633
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
BUTANEDIOLS
BIOLOGICAL EFFECTS
ETHANOL
FRUCTOSE
GLUCOSE
METABOLISM
BIOCHEMICAL REACTION KINETICS
CARBON 14 COMPOUNDS
CHICKENS
CLEARANCE
MONKEYS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALCOHOLS
ALDEHYDES
ANIMALS
BIRDS
CARBOHYDRATES
FOWL
GLYCOLS
HEXOSES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
KINETICS
LABELLED COMPOUNDS
MAMMALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PRIMATES
REACTION KINETICS
SACCHARIDES
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
550501 - Metabolism- Tracer Techniques