Studies on the biosynthesis and intracellular transport of gangliosides
Ganglioside biosynthesis and transport to myelin was studied in brainstem of 17-21 day old rats. Brainstem slices were incubated for up to 2 hours with (/sup 3/H)glucosamine, and gangliosides were isolated by column chromatography and HPTLC. Results from these experiments showed that: (a) ganglioside synthesis was decreased in the slices compared to in vivo, and this decrease was greater in the more complex gangliosides than in the simpler ones; (b) label incorporation into gangliosides GM3 and GM2 increased in a linear fashion, whereas the rate of incorporation continuously increased over the 2 hour period for the more complex gangliosides; (c) label incorporated into gangliosides, which showed almost no effect of chase after 30 minutes; (d) monensin at 0.1 uM inhibited the synthesis of all gangliosides except GM3, GM2 and GD3. Compartmentation of ganglioside biosynthesis was examined by analyzing the subcellular location of two ganglioside synthesizing enzymes, lactosylceramide sialosyltransferase (LCST) and GDlb sialosyltransferase (GDlbST), acting early and late in the ganglioside pathway, respectively.
- Research Organization:
- Wayne State Univ., Detroit, MI (USA)
- OSTI ID:
- 6101362
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMALS
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
BODY
BRAIN
CARBOHYDRATES
CENTRAL NERVOUS SYSTEM
CHROMATOGRAPHY
DISTRIBUTION
ENZYME ACTIVITY
ENZYMES
GANGLIOSIDES
GLUCOSAMINE
GLYCOLIPIDS
HEXOSAMINES
HEXOSES
ISOTOPE APPLICATIONS
LIPIDS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MEMBRANE TRANSPORT
MONOSACCHARIDES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
RATS
RODENTS
SACCHARIDES
SEPARATION PROCESSES
SUBCELLULAR DISTRIBUTION
SYNTHESIS
TRACER TECHNIQUES
TRANSFERASES
VERTEBRATES