Synthesis of gangliosides by cultured oligodendrocytes
Gangliosides are enriched in the nervous system compared to other tissues. The synthesis of gangliosides by monolayer cultures of isolated oligodendrocytes has not previously been investigated. Cells were labeled with (3H) galactose at preselected times and gangliosides isolated by phase partition, purified, and identified by chromatography. Cultured oligodendrocytes showed selectivity in their synthesis of gangliosides, which was expressed in the type of ganglioside synthesized as well as in the change of incorporation over time in culture. For the first ten days, there was very little incorporation of (3H) galactose in gangliosides, but this was followed by a stimulation of uptake for GM3, GM1/GD3, and GD1 gangliosides, reaching a maximum after approximately 25-30 days in vitro. There was little incorporation into GM2 or trisialogangliosides throughout the life of the cultures. Since oligodendrocytes synthesize extensive membranes during this period, one may speculate that the de novo-synthesized gangliosides are used for membranes.
- Research Organization:
- Hearst Laboratory of Neuroscience, Brain Research Institute, University of Chicago
- OSTI ID:
- 5551023
- Journal Information:
- J. Neurosci. Res.; (United States), Journal Name: J. Neurosci. Res.; (United States) Vol. 6:3; ISSN JNRED
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550301 -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMAL CELLS
BIOSYNTHESIS
CARBOHYDRATES
CELL CULTURES
GALACTOSE
GANGLIOSIDES
HEXOSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
MONOSACCHARIDES
NERVE CELLS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
SACCHARIDES
SOMATIC CELLS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS