UV-B-inducible and temperature-sensitive photoreactivation of cyclobutane pyrimidine dimers in Arabidopsis thaliana
- Oregon State Univ., Corvallis (United States)
Removal of cyclobutane pyrimidine dimers (CBPDs) in vivo from the DNA of UV-irradiated eight-leaf seedlings of Arabidopsis thaliana was rapid in the presence of visible light (half-life about 1 hour); removal of CBPDs in the dark, presumably via excision repair, was an order of magnitude slower. Extracts of plants contained significant photolyase in vitro, as assayed by restoration of transforming activity to UV-irradiated Escherichia coli plasmids; activity was maximal from four-leaf to 12-leaf stages. UV-B treatment of seedlings for 6 hours increased photolyase specific activity in extracts twofold. Arabidopsis photolyase was markedly temperature-sensitive, both in vitro (half-life at 30C about 12 minutes) and in vivo (half-life at 30C, 30 to 45 minutes). The wavelength dependency of the photoreactivation cross-section showed a broad peak at 375 to 400 nm, and is thus similar to that for maize pollen; it overlaps bacterial and yeast photolyase action spectra.
- OSTI ID:
- 6098544
- Journal Information:
- Plant Physiology; (United States), Journal Name: Plant Physiology; (United States) Vol. 95:2; ISSN 0032-0889; ISSN PLPHA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Inhibition of antigen-presenting activity of dendritic cells resulting from UV irradiation of murine skin is restored by in vitro photorepair of cyclobutane pyrimidine dimers
Photoreactivation of pyrimidine dimers in the DNA of normal and xeroderma pigmentosum cells
Related Subjects
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ARABIDOPSIS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DNA REPAIR
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYMES
FAR ULTRAVIOLET RADIATION
LYASES
MAGNOLIOPHYTA
MAGNOLIOPSIDA
ORGANIC COMPOUNDS
PHOTOREACTIVATION
PLANTS
PROTEINS
PYRIMIDINE DIMERS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SEEDLINGS
TEMPERATURE DEPENDENCE
ULTRAVIOLET RADIATION