Molecular mechanism of extinction of liver-specific functions in mouse hepatoma x rat fibroblast hybrids: extinction of the albumin gene

Journal Article · Fri Jan 01 04:00:00 EST 1982 · Somatic Cell Genet.; (United States)

DOI:https://doi.org/10.1007/BF01538893· OSTI ID:6097932

Papaconstantinou, J ^[1]; Wong, E; Ratrie, H; Szpirer, C; Szpirer, J

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1. Oak Ridge National Lab., TN

Hybrids formed by the fusion of mouse hepatoma (BWTG3) and rat fibroblast (JF1) cells exhibit the extinction of mouse albumin and ..cap alpha..-fetoprotein synthesis. Karyotype analyses suggest that all parental chromosomes are present in the hybrids. The extinction, therefore, of mouse hepatocyte genes is attributed to the inhibitory action of the rat genome. In these studies, we show that these hybrids possess and express the mouse ..beta..-glucyronidase gene (which is encoded on the same chromosome as the mouse albumin and ..cap alpha..-fetoprotein gene), and we present data of Southern blot analysis which demonstrate that such hybrids have indeed retained both mouse and rat albumin DNA sequences. In addition, using mouse albumin cDNA, we have shown by cDNA-RNA reassociation kinetics that albumin mRNA is virtually absent in these hybrids. We conclude from these studies that the extinction of albumin synthesis involves a mechanism which results in the loss of cytoplasmic albumin mRNA.

• OSTI ID: 6097932
• Journal Information: Somatic Cell Genet.; (United States), Journal Name: Somatic Cell Genet.; (United States) Vol. 8:3; ISSN SCEGD
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ANIMAL CELLS
ANIMALS
BIOSYNTHESIS
CONNECTIVE TISSUE CELLS
DATA
DISEASES
DNA
ENZYME ACTIVITY
ENZYMES
EXPERIMENTAL DATA
FIBROBLASTS
GENES
GLUCURONIDASE
GLYCOSYL HYDROLASES
HEPATOMAS
HYBRIDIZATION
HYDROLASES
INFORMATION
KARYOTYPE
MAMMALS
MESSENGER-RNA
MICE
NEOPLASMS
NUCLEIC ACIDS
NUMERICAL DATA
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PROTEINS
RATS
RNA
RODENTS
SOMATIC CELLS
SYNTHESIS
TEMPERATURE DEPENDENCE
TUMOR CELLS
VERTEBRATES