Isolation of a recombination-deficient mutant of Rhodopseudomonas capsulata

Journal Article · Sat Dec 01 04:00:00 EST 1984 · J. Bacteriol.; (United States)

OSTI ID:6096174

Genthner, F J; Wall, J D

To facilitate genetic analysis in the purple photosynthetic bacterium Rhodopseudomonas capsulata, a recombination-deficient derivative was sought. A UV irradiation-sensitive mutant (FG106F) was isolated after mutagenesis, and two procedures were used to determine the recombinational capacity of the mutant. First, recombinants were not detected after transduction of this derivative by the phage-like vector gene transfer agent. Second, an R-prime plasmid containing appropriately marked genes for photosynthesis was introduced by conjugation, and again no recombinants were observed. Additional phenotypes displayed by the mutant that are characteristic of a defect in recombination were an increased sensitivity to DNA-damaging antibiotics and a tendency to filament.

• Research Organization: Univ. of Missouri, Columbia
• OSTI ID: 6096174
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 160:3; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOLOGY
CELL CONSTITUENTS
CHEMICAL REACTIONS
ELECTROMAGNETIC RADIATION
GENE RECOMBINATION
GENES
GENETICS
MICROORGANISMS
MUTAGENESIS
MUTANTS
PHENOTYPE
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PLASMIDS
RADIATIONS
RHODOPSEUDOMONAS
SENSITIVITY
SYNTHESIS
ULTRAVIOLET RADIATION