Degradation of o-nitrophenol and m-nitrophenol by a Pseudomonas putida
Journal Article
·
· J. Agric. Food Chem.; (United States)
A Pseudomonas putida strain that grew on o-nitrophenol (ONP) and m-nitrophenol (MNP) as sole sources of carbon and nitrogen was isolated from soil. The microorganism was unable to metabolize p-nitrophenol. Growing and resting cells of P. putida degraded ONP releasing nitrite and MNP releasing ammonium. Enzymes involved in the metabolism of ONP or MNP were inducible by ONP or MNP, respectively. ONP was degradable in a cell-free system. The initial enzyme converted ONP to catechol and nitrite. The enzyme required NADPH as a coenzyme and its specific activity was 15 ..mu..mol min/sup -1/ (g of protein)/sup -1/. Catechol was further degraded through the ortho-cleavage pathway to ..beta..-ketoadipic acid. Attempts to demonstrate the degradation of MNP in a cell-free system failed and the pathway of the MNP metabolism remains unknown. 25 references, 3 figures, 5 tables.
- Research Organization:
- Environmental Quality Inst., Beltsville, MD
- OSTI ID:
- 6094925
- Journal Information:
- J. Agric. Food Chem.; (United States), Journal Name: J. Agric. Food Chem.; (United States) Vol. 32:2; ISSN JAFCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMMONIUM COMPOUNDS
AROMATICS
BACTERIA
BIODEGRADATION
BIOLOGICAL PATHWAYS
CHEMICAL REACTIONS
COENZYMES
DATA
DECOMPOSITION
DEVELOPERS
ENZYME ACTIVITY
EXPERIMENTAL DATA
HYDROXY COMPOUNDS
INFORMATION
METABOLISM
MICROORGANISMS
NITRITES
NITRO COMPOUNDS
NITROGEN COMPOUNDS
NITROPHENOL
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PHENOLS
POLYPHENOLS
PSEUDOMONAS
PYROCATECHOL
SOILS
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMMONIUM COMPOUNDS
AROMATICS
BACTERIA
BIODEGRADATION
BIOLOGICAL PATHWAYS
CHEMICAL REACTIONS
COENZYMES
DATA
DECOMPOSITION
DEVELOPERS
ENZYME ACTIVITY
EXPERIMENTAL DATA
HYDROXY COMPOUNDS
INFORMATION
METABOLISM
MICROORGANISMS
NITRITES
NITRO COMPOUNDS
NITROGEN COMPOUNDS
NITROPHENOL
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PHENOLS
POLYPHENOLS
PSEUDOMONAS
PYROCATECHOL
SOILS