Pathway for biodegradation of p-nitrophenol in a Moraxella sp
- Air Force Engineering and Services Center, Tyndall AFB, FL (USA)
- Univ. of Iowa, Iowa City (USA)
A Moraxella strain grew on p-nitrophenol with stoichiometric release of nitrite. During induction of the enzymes for growth on p-nitrophenol, traces of hydroquinone accumulated in the medium. In the presence of 2,2{prime}-dipyidyl, p-nitrophenol, was converted stoichiometrically to hydroquinone. Particulate enzymes catalyzed the conversion of p-nitrophenol to hydroquinone in the presence of NADPH and oxygen. Soluble enzymes catalyzed the conversion of hydroquinone to {gamma}-hydroxymuconic semialdehyde, which was identified by high-performance liquid chromatography (HPLC)-mass spectroscopy. Upon addition of catalytic amounts of NAD{sup +}, {gamma}-hydroxymuconic semialdehyde was converted to {beta}-ketoadipic acid. In the presence of pyruvate and lactic dehydrogenase, substrate amounts of NAD were required and {gamma}-hydroxymuconic semialdehyde was converted to maleylacetic acid, which was identified by HPLC-mass spectroscopy. Similar results were obtained when the reaction was carried out in the presence of potassium ferricyanide. Extracts prepared from p-nitrophenol-grown cells also contained an enzyme that catalyzed the oxidation of 1,2,4-benzenetriol to maleylacetic acid. The enzyme responsible for the oxidation of 1,2,4-benzenetriol was separated from the enzyme responsible for hydroquinone oxidation by DEAE-cellulose chromatography. The results indicate that the pathway for biodegradation of p-nitrophenol involves the initial removal of the nitro group as nitrite and formation of hydroquinone.
- OSTI ID:
- 5294703
- Journal Information:
- Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 57:3; ISSN 0099-2240; ISSN AEMID
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
090900* -- Biomass Fuels-- Processing-- (1990-)
54 ENVIRONMENTAL SCIENCES
540320 -- Environment
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550300 -- Cytology
59 BASIC BIOLOGICAL SCIENCES
AROMATICS
BACTERIA
BIODEGRADATION
BIOLOGICAL PATHWAYS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DECOMPOSITION
ENZYMES
FRACTIONATION
HYDROXY COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MICROORGANISMS
NITRITES
NITRO COMPOUNDS
NITROGEN COMPOUNDS
NITROPHENOL
NUCLEOTIDE DEHYDROGENASES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PHENOLS
SEPARATION PROCESSES