Potentiation of butylated hydroxytoluene-induced acute lung damage by oxygen: cell kinetics and collagen

Journal Article · Sat Jan 01 04:00:00 EST 1983 · Am. Rev. Respir. Dis.; (United States)

DOI:https://doi.org/10.1164/arrd.1983.127.1.28· OSTI ID:6093272

Haschek, W M; Reiser, K M; Klein-Szanto, A J.P.; Kehrer, J P; Smith, L H; Last, J A; Witschi, H P

Changes in cell proliferation and in collagen synthesis were studied in young adult male BALB/c mice injected intraperitoneally with 400 mg/kg of butylated hydroxytoluene (BHT) in corn oil or corn oil alone and immediately exposed to 70% oxygen or air for 6 days. Mice received (/sup 3/H)thymidine either as a single injection 90 min before being killed or as a continual infusion via an osmotic minipump. Autoradiography was done 2 to 14 days after BHT injection, and cell kinetic studies were performed. In a similar experiment, mice were injected intraperitoneally with (/sup 3/H)proline 3 h before being killed, and type I/type III collagen ratio in newly synthesized lung collagen was determined. It was found that exposure to 70% oxygen immediately after the administration of BHT initially delayed the epithelial cell proliferation and the decrease in the percentage of newly synthesized type III collagen that occurred after BHT alone. Once the animals were removed from oxygen there was a compensatory burst of cell proliferation and a precipitous drop in the percentage of newly synthesized type III collagen. The proliferating cell population after removal from oxygen was primarily interstitial and not epithelial. When exposure to oxygen was delayed, cell proliferation was similar to that seen after BHT injection alone.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 6093272
• Journal Information: Am. Rev. Respir. Dis.; (United States), Journal Name: Am. Rev. Respir. Dis.; (United States) Vol. 127; ISSN ARRDA
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDITIVES
AMINES
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
ANTIOXIDANTS
AUTORADIOGRAPHY
AZINES
AZOLES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL MODELS
BIOSYNTHESIS
BODY
CARBOXYLIC ACIDS
CELL PROLIFERATION
COLLAGEN
ELEMENTS
EPITHELIUM
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
HYDROXYPROLINE
INJECTION
INJURIES
INTAKE
INTRAPERITONEAL INJECTION
KINETICS
LABELLED COMPOUNDS
LUNGS
MAMMALS
MICE
NONMETALS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXYGEN
PROTEINS
PYRIMIDINES
PYRROLES
PYRROLIDINES
REACTION KINETICS
RESPIRATORY SYSTEM
RIBOSIDES
RODENTS
SCLEROPROTEINS
SYNTHESIS
THYMIDINE
TISSUES
TOXICITY
TRITIUM COMPOUNDS
VARIATIONS
VERTEBRATES