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Modulation of carbachol-stimulated inositol phospholipid hydrolysis in rat cerebral cortex

Journal Article · · Neurochem. Res.; (United States)
DOI:https://doi.org/10.1007/BF00970524· OSTI ID:6087155
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Cortical slices from rat brain were used to study carbachol-stimulated inositol phospholipid hydrolysis. Omission of calcium during incubation of slices with (/sup 3/H)inositol increased its incorporation into receptor-coupled phospholipids. Carbachol-stimulated hydrolysis of (/sup 3/H)inositol phospholipids in slices was dose-dependent, was affected by the concentrations of calcium and lithium present and resulted in the accumulation of mostly (/sup 3/H)inositol-1-phosphate. Incubation of slices with N-ethylmaleimide or a phorbol ester reduced the response to carbachol. Membranes prepared from cortical slices labeled with (/sup 3/H)inositol retained the receptor-stimulated inositol phospholipid hydrolysis reaction. The basal rate of inositol phospholipid hydrolysis was higher than in slices and addition of carbachol further stimulated the process. Addition of GTP stimulated inositol phospholipid hydrolysis, suggesting the presence of a guanine nucleotide-binding protein coupled to phospholipase C. Carbachol and GTP-stimulated inositol phospholipid hydrolysis in membranes was detectable following a 3 min assay period. In contrast to slices, increased levels of inositol bisphosphate and inositol trisphosphate were detected following incubation of membranes with carbachol. These results demonstrate that agonist-responsive receptors are present in cortical membranes, that the receptors may be coupled to phosphatidylinositol 4, 5-bisphosphate, rather than phosphatidylinositol, hydrolysis and that a guanine nucleotide-binding protein may mediate the coupling of receptor activation to inositol phospholipid hydrolysis in brain.

Research Organization:
Univ. of Alabama, Birmingham
OSTI ID:
6087155
Journal Information:
Neurochem. Res.; (United States), Journal Name: Neurochem. Res.; (United States) Vol. 12:8; ISSN NERED
Country of Publication:
United States
Language:
English

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Related Subjects

550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METALS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
BRAIN
CARBOHYDRATES
CARCINOGENS
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM
CEREBRAL CORTEX
CEREBRUM
CHLORIDES
CHLORINE COMPOUNDS
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELEMENTS
ESTERS
HALIDES
HALOGEN COMPOUNDS
IN VITRO
INOSITOL
INOSITOLS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIPIDS
LITHIUM
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
METABOLISM
METALS
MONOSACCHARIDES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PARASYMPATHOMIMETICS
PHORBOL ESTERS
PHOSPHOLIPIDS
PROTEINS
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SACCHARIDES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES