Solubilization and functional reconstitution of polymorphonuclear leukocyte formyl-Methionyl-Leucyl-Phenylalanine receptors and guanine nucleotide binding proteins
Formyl-Methionyl-Leucyl-Phenylalanine (fMLP) binds to specific polymorphonuclear leukocyte plasma membrane receptors stimulating chemotaxis and bactericidal responses. One of the initial events of the ligand receptor interaction is a rise in inositol trisphosphate, which triggers intracellular calcium release. The generation of inositol trisphosphate is mediated by the fMLP-activated phospholipase C via a GTP-binding protein (G-protein). In analogy to the adrenergic stimulation of adenylate cyclase, the following signal transduction model has been proposed: The fMLP receptor activates a G-protein which then stimulates phospholipase C to hydrolyse phosphatidylinositol bisphosphate to inositol trisphosphate and diacylglycerol. This work has focused on characterizing the structural and functional coupling fMLP receptor and G-proteins in native membranes, detergent micelles and reconstituted phospholipid vesicles. Tight coupling between the fMLP receptor and G-protein has been demonstrated in both native and solubilized membranes by assaying quanine nucleotide-induced inhibition of (/sup 3/H)fMLP binding and fMLP stimulated GTPase activity.
- Research Organization:
- Boston Univ., MA (USA)
- OSTI ID:
- 6974765
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACIDS
AROMATICS
AZAARENES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
ENZYME ACTIVITY
ENZYMES
ESTERASES
ESTERS
GUANINE
HETEROCYCLIC COMPOUNDS
HYDROLASES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LEUKOCYTES
LIPASE
LIPIDS
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHENYLALANINE
PHOSPHOLIPIDS
PROTEINS
PURINES
REACTION KINETICS
RECEPTORS
SOLUBILITY
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS