Binding, sequestration, and processing of epidermal growth factor and nerve growth factor by PC12 cells. [Rats]

Journal Article · Tue Mar 01 04:00:00 EST 1983 · J. Cell. Physiol.; (United States)

DOI:https://doi.org/10.1002/jcp.1041140311· OSTI ID:6086431

Chandler, C E; Herschman, H R

Th rat PC12 pheochromocytoma cell line exhibits biological responses to both nerve growth factor (NGF) and epidermal growth factor (EGF). The existence of receptors and biological responses on a common cell for these two well-characterized polypeptide growth factors makes this an attractive system for comparison of ligand binding and processing. Both NGF and EGF are bound to PC12 cells in a competable form at 4/sup 0/C. At 37/sup 0/C both ligands are ''sequestered,'' but at different rates and to different extents. While sequestration happens rapidly and nearly quantitatively for bound EGF, the dissociation reaction appears to compete favorably with NFG sequestration. Both EGF and NGF are degraded by PC12 cells. Sequestered EGF, however, is degraded to a greater extent than sequestered NGF.

• Research Organization: Univ. of California School of Medicine, Los Angeles
• DOE Contract Number: AC03-76SF00012
• OSTI ID: 6086431
• Journal Information: J. Cell. Physiol.; (United States), Journal Name: J. Cell. Physiol.; (United States) Vol. 114:3; ISSN JCLLA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
551000 -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
BIOCHEMISTRY
BIOLOGICAL FUNCTIONS
BODY
CHEMISTRY
EPIDERMIS
EPITHELIUM
FUNCTIONS
LIGANDS
MORPHOLOGICAL CHANGES
NERVE CELLS
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
POLYPEPTIDES
PROTEINS
RECEPTORS
SKIN
SOMATIC CELLS
TISSUES