Epidermal growth factor receptors on PC12 cells: alteration of binding properties by lectins

Journal Article · Sat Jan 01 04:00:00 EST 1983 · J. Cell. Biochem.; (United States)

DOI:https://doi.org/10.1002/jcb.240220204· OSTI ID:6832795

Vale, R D; Shooter, E M

The PC12 cell line displays cell surface receptors for both nerve growth factor (NGF) and epidermal growth factor (EGF). It has been previously shown that the lectin wheat germ agglutinin (WGA) alters the properties of NGF receptors on these cells. We now report that preincubations with either WGA or concanavalin A (Con A) decrease the binding of /sup 125/I-EGF to PC12 cells by greater than 50%. The inhibition of binding occurred at 37 degrees C and 4 degrees C and could be blocked or reversed by the addition of sugars which bind specifically to WGA or Con A. Scatchard analysis revealed that these lectins decreased binding primarily by lowering the affinity of the receptor and to a lesser extent by decreasing receptor number. Succinylation of Con A (sCon A) produced a derivative that was less effective than the native lectin in decreasing EGF binding; however, addition of an antibody against Con A restored the ability of sCon A to decrease binding. Similar to results obtained with /sup 125/I-NGF binding, WGA but not Con A was found to increase, by severalfold, the proportion of /sup 125/I-EGF binding that is resistant to solubilization by Triton X-100 detergent. A potential association of the EGF receptor with cytoskeletal elements is discussed which could account for such results.

• Research Organization: Department of Neurobiology, Stanford University School of Medicine, California
• OSTI ID: 6832795
• Journal Information: J. Cell. Biochem.; (United States), Journal Name: J. Cell. Biochem.; (United States) Vol. 22:2; ISSN JCEBD
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CHEMICAL BONDS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EPIDERMIS
EPITHELIUM
GROWTH
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LECTINS
NERVE CELLS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOISOTOPES
RECEPTORS
SKIN
SOMATIC CELLS
STH
TISSUES
TRACER TECHNIQUES