Epoxidation of prostacyclin in the rabbit kidney

Journal Article · Mon Aug 05 04:00:00 EDT 1985 · J. Biol. Chem.; (United States)

OSTI ID:6085905

Wong, P Y; Malik, K U; Taylor, B M; Schneider, W P; McGiff, J C; Sun, F F

In the isolated Tyrode's perfused rabbit kidney, metabolism of (9-TH)prostacyclin was examined. In addition to 7,9-dihydroxy-4,13-diketo-dinor-prostanoic acid, dinor-6-keto-prostaglandin F1 alpha, and pentanorprostaglandin (PG)F1 alpha gamma-lactone, a new, previously unreported, metabolite was isolated and identified by radio-gas chromatography and gas chromatography-mass spectrometry as 5-hydroxy-6-keto-PGF1 alpha. The structure of this metabolite was further confirmed by comparison of the mass spectra to that of the synthetic standard. The formation of 5-hydroxy-6-keto-PGF1 alpha in the kidney suggested epoxidation of prostacyclin via the renal epoxygenase pathway.

• Research Organization: New York Medical College, Valhalla
• OSTI ID: 6085905
• Journal Information: J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 16; ISSN JBCHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BODY
CHEMICAL REACTIONS
CHROMATOGRAPHY
GAS CHROMATOGRAPHY
KIDNEYS
LABELLED COMPOUNDS
MAMMALS
MASS SPECTRA
METABOLITES
ORGANS
OXIDATION
PROSTAGLANDINS
RABBITS
RADIOCHROMATOGRAPHY
SEPARATION PROCESSES
SPECTRA
TRITIUM COMPOUNDS
VERTEBRATES