PGI2 synthesis and excretion in dog kidney: evidence for renal PG compartmentalization

Journal Article · Wed Jan 01 04:00:00 EST 1986 · Am. J. Physiol.; (United States)

OSTI ID:5942673

Boyd, R M; Nasjletti, A; Heerdt, P M; Baer, P G

To assess the concept of compartmentalization of renal prostaglandins (PG), we compared entry of PGE2 and the PGI2 metabolite 6-keto-PGF1 alpha into the renal vascular and tubular compartments, in sodium pentobarbital-anesthetized dogs. Renal arterial 6-keto-PGF1 alpha infusion increased both renal venous and urinary 6-keto-PGF1 alpha outflow. In contrast, renal arterial infusion of arachidonic acid (AA) or bradykinin (BK) increased renal venous 6-keto-PGF1 alpha outflow but had no effect on its urinary outflow. Both urinary and renal venous PGE2 outflows increased during AA or BK infusion. Ureteral stopped-flow studies revealed no postglomerular 6-keto-PGF1 alpha entry into tubular fluid. During renal arterial infusion of (3H)PGI2 and inulin, first-pass 3H clearance was 40% of inulin clearance; 35% of urinary 3H was 6-keto-PGF1 alpha, and two other urinary metabolites were found. During renal arterial infusion of (3H)6-keto-PGF1 alpha and inulin, first-pass 3H clearance was 150% of inulin clearance; 75% of urinary 3H was 6-keto-PGF1 alpha, and only one other metabolite was found. We conclude that in the dog PGE2 synthesized in the kidney enters directly into both the renal vascular and tubular compartments, but 6-keto-PGF1 alpha of renal origin enters directly into only the renal vascular compartment.

• Research Organization: Univ. of Tennessee Center for the Health Sciences, Memphis
• OSTI ID: 5942673
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 1; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BODY FLUIDS
BRADYKININ
CARBOHYDRATES
CLEARANCE
DOGS
EXCRETION
INULIN
KININS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
PEPTIDES
POLYPEPTIDES
POLYSACCHARIDES
PROSTAGLANDINS
PROTEINS
RENAL CLEARANCE
SACCHARIDES
SYNTHESIS
TRITIUM COMPOUNDS
VERTEBRATES