Profile of the intestinal mucosal corticosteroid receptors in the domestic duck

Journal Article · Mon Jul 01 00:00:00 EDT 1985 · Gen. Comp. Endocrinol.; (United States)

DOI:https://doi.org/10.1016/0016-6480(85)90416-2· OSTI ID:6084355

DiBattista, J A; Mehdi, A Z; Sandor, T

The corticosteroid receptor profile of the intestinal tract of the domestic duck (maintained on either a low-sodium (LS) or a high-sodium (HS) diet) was investigated. Using tritiated triamcinolone acetonide (TA), corticosterone, or aldosterone as ligands, cytoplasmic mineralocorticoid receptors (MR, type I) and glucocorticoid receptors (GR, type II) were found in the mucosal cytosol of the jejunum and colon. The diet little influenced the GR binding parameters, while the MR (aldosterone) binding parameters showed a down-regulation following LS diets. The competition hierarchy of radioinert steroids on the formation of the (TH)corticosterone-receptor complex was corticosterone = cortisol = 11-deoxycorticosterone greater than aldosterone = TA = dexamethasone much greater than 11-deoxycortisol; with (TH)aldosterone, the competition was corticosterone = progesterone = 11-deoxycorticosterone greater than aldosterone = cortisol = TA = dexamethasone greater than 11-deoxycortisol greater than 11-dehydrocorticosterone. On linear sucrose gradients, receptor-ligand complexes sedimented with a single peak at 8.5 S (hypotonic gradient) and 4.0-4.5 S (hypertonic gradient), respectively. Heat-activated (TH)TA- and (TH)aldosterone-receptor complexes bound avidly to DNA-cellulose and, upon ion-exchange chromatography on DEAE-Sephacel, the presence of the negatively charged unactivated and the more positively charged activated complexes could be shown.

• Research Organization: Hopital Notre-Dame, Montreal, Quebec, Canada
• OSTI ID: 6084355
• Journal Information: Gen. Comp. Endocrinol.; (United States), Vol. 1
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
CORTICOSTEROIDS
RADIORECEPTOR ASSAY
INTESTINES
BIOCHEMICAL REACTION KINETICS
ALDOSTERONE
CORTICOSTERONE
DNA
DUCKS
ENZYMES
MUCOUS MEMBRANES
RECEPTORS
TRITIUM COMPOUNDS
ADRENAL HORMONES
ALDEHYDES
ANIMALS
BIRDS
BODY
DIGESTIVE SYSTEM
FOWL
GASTROINTESTINAL TRACT
GLUCOCORTICOIDS
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
KINETICS
LABELLED COMPOUNDS
MEMBRANES
MINERALOCORTICOIDS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PREGNANES
REACTION KINETICS
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics