Determination of selenium in small biological samples by graphite furnace atomic absorption spectrometry

Miscellaneous · Sun Jan 01 00:00:00 EST 1989

OSTI ID:6082383

Martin, C K

Initial work on developing a method for selenium determination in biological samples was conducted using a graphite furnace atomic absorption spectrometer with deuterium-background-correction. This instrument was inadequate for the task and was replaced with a Zeeman background-corrected spectrometer. A method for the determination of biological selenium was developed using Zeeman graphite furnace atomic absorption spectrometry with calibration against aqueous standards. Data is presented on the recovery of selenium from NBS Bovine Liver 1577a. A microwave digestion method using nitric acid was applied to samples weighing only a few milligrams. A novel matrix modifier system was employed which caused biological selenium to behave like inorganic selenium standards with regard to appearance time and peak shape. This system was a 1:1 v/v mixture of 1200 mg/L palladium nitrate and 1000 mg/L copper nitrate used as the modifier solution, and 0.1% Triton X-100 added to standards and samples. The overall average selenium recovery from 0.005-0.650 gram samples of the liver SRM was 98.1 {plus minus} 5.4% (n = 38) by direct calibration, and 101.4 {plus minus} 3.8% (n = 16) by standard additions calibration using this method.

• Research Organization: Memphis State Univ., TN (USA)
• OSTI ID: 6082383
• Resource Relation: Other Information: Thesis (Ph.D)
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
BIOLOGICAL MATERIALS
CHEMICAL ANALYSIS
SELENIUM
ABSORPTION SPECTROSCOPY
CALIBRATION
COPPER NITRATES
FURNACES
GRAPHITE
LIVER CELLS
MICROWAVE HEATING
PALLADIUM NITRATES
SAMPLE PREPARATION
SURFACTANTS
SWINE
ZEEMAN EFFECT
ANIMAL CELLS
ANIMALS
CARBON
COPPER COMPOUNDS
DOMESTIC ANIMALS
ELEMENTAL MINERALS
ELEMENTS
HEATING
MAMMALS
MATERIALS
MINERALS
NITRATES
NITROGEN COMPOUNDS
NONMETALS
OXYGEN COMPOUNDS
PALLADIUM COMPOUNDS
SEMIMETALS
SOMATIC CELLS
SPECTROSCOPY
TRANSITION ELEMENT COMPOUNDS
VERTEBRATES
400102* - Chemical & Spectral Procedures