Comparison of isotope dilution mass spectrometry and graphite furnace atomic absorption spectrometry with Zeeman background correction for determination of plasma selenium
There has been increasing interest in the measurement of selenium in plasma because of the toxicological and nutritional importance of selenium. More recently selenium has been implicated as an anticarcinogen and a carcino-preventive agent. Several techniques are used to measure plasma selenium concentrations. One of the most popular, at present, is the use of graphite furnace atomic absorption spectrometry with Zeeman background correction (ZAAS), which permits the determination of selenium in plasma with minimal sample manipulation. Plasma is diluted with a matrix modifier containing Ni and Mg to thermally stabilize selenium and use a L'vov platform and peak area integration. However, this method is validated using an isotope dilution mass spectrometry (IDMS) technique, which can be considered a definitive method. Comparison of results obtained by two independent methods that utilize different physical properties is probably the best way to verify the accuracy of a new method. Imprecisions for both methods are established by the regular use of well-characterized reference materials. 7 references, 2 tables.
- Research Organization:
- Dept. of Agriculture, Beltsville, MD
- OSTI ID:
- 5699662
- Journal Information:
- Anal. Chem.; (United States), Vol. 58:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
PLASMA
QUANTITATIVE CHEMICAL ANALYSIS
SELENIUM
ABSORPTION SPECTROSCOPY
ISOTOPE DILUTION
MASS SPECTROSCOPY
ACCURACY
COMPARATIVE EVALUATIONS
EXPERIMENTAL DATA
MAGNESIUM
NICKEL
ZEEMAN EFFECT
ALKALINE EARTH METALS
CHEMICAL ANALYSIS
DATA
ELEMENTS
INFORMATION
ISOTOPE APPLICATIONS
METALS
NUMERICAL DATA
SEMIMETALS
SPECTROSCOPY
TRACER TECHNIQUES
TRANSITION ELEMENTS
400104* - Spectral Procedures- (-1987)