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Characterization and applications of monoclonal antibodies to the prolactin receptor

Journal Article · · Endocrinology; (United States)
; ; ;
Monoclonal antibodies (mAbs) were produced in BALB/c mice immunized with partially purified PRL receptors from rat liver. Two mAbs (T1 and T6) were able to completely inhibit (125I)ovine PRL ((125I)oPRL) binding to solubilized rat liver PRL receptors, while two other mAbs (U5 and U6) showed only a small effect on PRL binding, but were able to precipitate hormone-receptor complexes. Scatchard analysis of (125I)oPRL binding to rat liver microsomes in the presence of mAbs resulted in a decrease in the number of sites without changing the affinity of PRL binding by T1 and T6, whereas U5 and U6 altered neither parameter. (125I)mAb binding to rat liver microsomes was performed in the presence of various concentrations of unlabeled mAbs or oPRL to examine the interaction between mAbs. Competition of binding to the receptor was observed, respectively, between T1 and T6, U5 and U6, and U5 and E21 (a mAb to the rat liver PRL receptor previously produced). Both (125I)T1 and (125I)T6 binding were inhibited by oPRL, although not completely (80% inhibition at the higher concentrations). When (125I)T1 binding was analyzed by Scatchard analysis, two classes of binding sites to rat liver microsomes were found, of which only the number of higher affinity sites was affected by the presence of oPRL in incubation. Similar results were observed for (125I)T6 binding. (125I)mAb binding to microsomes from other tissues and species was examined. All five mAbs were able to bind to microsomes from rat tissues (liver, ovary, adrenal, prostate, and Nb2 lymphoma cells), similar to the level of (125I)oPRL binding in these tissues. The binding characteristics of (125I)T6 or (125I)U5 were essentially identical in all rat tissues examined.
Research Organization:
McGill Univ., Montreal, Quebec (Canada)
OSTI ID:
6081027
Journal Information:
Endocrinology; (United States), Journal Name: Endocrinology; (United States) Vol. 124:5; ISSN ENDOA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CHEMICAL COMPOSITION
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
GLANDS
GONADOTROPINS
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIVER
LTH
MAMMALS
MEMBRANE PROTEINS
MICE
MONOCLONAL ANTIBODIES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
RABBITS
RADIOISOTOPES
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SPECIFICITY
SWINE
TRACER TECHNIQUES
VERTEBRATES