Prolactin receptors in liver, kidney, and gill of the tilapia (Oreochromis mossambicus): Characterization and effect of salinity on specific binding of iodinated ovine prolactin

Journal Article · Thu Mar 01 04:00:00 EST 1990 · General and Comparative Endocrinology; (USA)

DOI:https://doi.org/10.1016/0016-6480(90)90226-C· OSTI ID:6747132

Dauder, S; Young, G; Hass, L; Bern, H A ^[1]

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1. Univ. of California, Berkeley (USA)

Specific binding of {sup 125}I-ovine prolactin (oPRL) to microsomal fractions from gill, kidney, and liver of adult tilapia was determined. Specific binding varied among tissues, the highest values being displayed by kidney membranes. In the liver, the binding of oPRL was not strongly displaced by tilapia prolactins (tPRL177 and tPRL188), although tPRL177 was six times more potent than tPRL188. On the other hand, in kidney and gill membranes, the two tPRLs were equipotent. Tilapia PRLs showed low potency in competing for oPRL-binding sites when pregnant rat liver membranes were utilized. Tilapia growth hormone (tGH) and human growth hormone (hGH) displaced {sup 125}I-oPRL from liver as well as did tPRL177 but were not recognized well by renal or branchial receptors. Two {sup 125}I-oPRL-binding sites were detected in every tissue tested. These binding sites are subject to physiological regulation since adaptation to seawater resulted in a significant decrease in specific binding.

• OSTI ID: 6747132
• Journal Information: General and Comparative Endocrinology; (USA), Journal Name: General and Comparative Endocrinology; (USA) Vol. 77:3; ISSN 0016-6480; ISSN GCENA
• Country of Publication: United States
• Language: English

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550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL COMPOSITION
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
FISHES
GILLS
GLANDS
GONADOTROPINS
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
KINETICS
LIVER
LTH
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
PREGNANCY
PROTEINS
RADIOASSAY
RADIOISOTOPES
RATS
REACTION KINETICS
RECEPTORS
RESPIRATORY SYSTEM
RODENTS
RUMINANTS
SHEEP
TRACER TECHNIQUES
VERTEBRATES