Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Specific binding of endothelin on human vascular smooth muscle cells in culture

Journal Article · · J. Clin. Invest.; (United States)
DOI:https://doi.org/10.1172/JCI114078· OSTI ID:6080958
; ;

Endothelin is a newly discovered, potent vasoconstrictor peptide secreted by endothelial cells. The binding of endothelin was studied on cultured human vascular smooth muscle cells obtained from umbilical veins. A single specific binding site for /sup 125/I-endothelin was identified, with an apparent Kd of 126 pM and a maximal binding capacity of approximately 10,000 sites per smooth muscle cell. At room temperature the binding was saturable, reached equilibrium at 2 h (using 20 pM endothelin), and was slowly and only partially reversed by unlabeled endothelin. The calcium antagonists nifedipine, nicardipine, and diltiazem did not compete for the same binding site. Conditioned medium from cultured human umbilical vein endothelial cells inhibited the binding of /sup 125/I-endothelin dose dependently. This effect was antagonized by anti-endothelin antiserum. We conclude that human umbilical vein smooth muscle cells possess specific binding sites for endothelin, and that human endothelial cells secrete an endothelinlike material.

Research Organization:
F. Hoffmann-La Roche and Co., Ltd., Basle (Switzerland)
OSTI ID:
6080958
Journal Information:
J. Clin. Invest.; (United States), Journal Name: J. Clin. Invest.; (United States) Vol. 83:5; ISSN JCINA
Country of Publication:
United States
Language:
English

Similar Records

Vascular mechanism of action of endothelin-1: Effect of Ca2+ antagonists
Journal Article · Sat Dec 31 23:00:00 EST 1988 · Journal of Cardiovascular Pharmacology; (USA) · OSTI ID:5405538
Human cultured endothelial cells do secrete endothelin-1
Journal Article · Sat Dec 31 23:00:00 EST 1988 · Journal of Cardiovascular Pharmacology; (USA) · OSTI ID:5564080
Down-regulation of endothelin binding sites in rat vascular smooth muscle cells
Journal Article · Sat Mar 31 23:00:00 EST 1990 · American Journal of Hypertension; (USA) · OSTI ID:6709257

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
BLOOD VESSELS
BODY
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
CELL CULTURES
CHEMISTRY
CULTURE MEDIA
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
INHIBITION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MAN
MEMBRANE PROTEINS
MUSCLES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
TISSUES
TRACER TECHNIQUES
VASOCONSTRICTORS
VEINS
VERTEBRATES