Human cultured endothelial cells do secrete endothelin-1

Journal Article · Sun Jan 01 04:00:00 EST 1989 · Journal of Cardiovascular Pharmacology; (USA)

DOI:https://doi.org/10.1097/00005344-198900135-00068· OSTI ID:5564080

Clozel, M; Fischli, W ^[1]

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1. F. Hoffmann-La Roche and Co., AG, Basel (Switzerland)

Endothelin-1 (ET-1) has been identified in the conditioned medium of porcine endothelial cells. Human endothelin (ET-1) cloned from a placenta cDNA library is similar to porcine, but it is not known whether endothelin itself is secreted by human endothelial cells. To answer this question, a conditioned medium taken every 48 h from confluent cultures of umbilical vein endothelial cells was analyzed by HPLC and all fractions were tested for their ability to inhibit ({sup 125}I)ET-1 binding on human placenta membranes. Only one fraction did inhibit ({sup 125}I)ET-1 binding. When the conditioned medium was spiked with ET-1, the same single fraction inhibited ({sup 125}I)ET-1 binding showing that ET-1, itself, is present in the conditioned medium of human endothelial cells. ET-1 accumulates with time, reaching a plateau at 48 h. ET-1 secretion is not increased by a 24-h incubation of endothelial cells with phorbol myristate acetate, interleukin-1, tumor necrosis factor, thrombin or neuropeptide Y.

• OSTI ID: 5564080
• Journal Information: Journal of Cardiovascular Pharmacology; (USA), Journal Name: Journal of Cardiovascular Pharmacology; (USA) Vol. 13 Suppl 5; ISSN 0160-2446; ISSN JCPCD
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETATES
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BLOOD VESSELS
BODY
CARBOXYLIC ACID SALTS
CARCINOGENS
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
CELL MEMBRANES
CHROMATOGRAPHY
COAGULANTS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
ENZYMES
ESTERS
FETAL MEMBRANES
GROWTH FACTORS
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
IN VITRO
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIQUID COLUMN CHROMATOGRAPHY
LYMPHOKINES
MAMMALS
MAN
MEMBRANES
MITOGENS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HYDROLASES
PEPTIDES
PHORBOL ESTERS
PLACENTA
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SECRETION
SEPARATION PROCESSES
SERINE PROTEINASES
THROMBIN
TISSUES
TRACER TECHNIQUES
VASOCONSTRICTION
VASOCONSTRICTORS
VEINS
VERTEBRATES