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Mutagenicity of algal metabolites of benzo(a)pyrene for Salmonella typhimurium

Journal Article · · Environ. Mutagen.; (United States)
; ; ;
The metabolism and growth effects of benzo(a)pyrene (BaP) were studied using a freshwater green alga, Selenastrum capricornutum. Algal cultures were incubated under gold light with BaP added at concentrations of 40, 160, 400, and 1200 g/liter for the periods of 1-4 days. The metabolites and BaP were identified and quantified from ethyl acetate extracts of both algal cells and incubation medium. The ethyl acetate extracts were evaluated for genotoxicity using a micro-volume Salmonella typhimurium forward mutation assay with resistance to 8-azaguanine for selection. This assay detected the presence of small quantities of BaP and was particularly sensitive to the mutagenicity of BaP diols. Of those extracts prepared from algae and medium from cultures exposed to 400 g BaP/liter (10 g/25 ml culture), only algal cell extracts from one day's growth were mutagenic. In cultures exposed to 1200 g BaP/liter (30 g/25 ml culture), mutagenic materials were produced or persisted in both algae and media throughout the 4-day incubation. The observed mutagenic response can be attributed in part to the presence of unmetabolized BaP or to BaP diols.
Research Organization:
Univ. of Cincinnati Medical Center, OH
OSTI ID:
6080778
Journal Information:
Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 7:6; ISSN ENMUD
Country of Publication:
United States
Language:
English

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Related Subjects

560302 -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
560303* -- Chemicals Metabolism & Toxicology-- Plants-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALGAE
AROMATICS
BACTERIA
BENZOPYRENE
BIOLOGICAL EFFECTS
CARBON 14 COMPOUNDS
CONDENSED AROMATICS
GENETIC EFFECTS
HYDROCARBONS
LABELLED COMPOUNDS
METABOLITES
MICROORGANISMS
MUTAGEN SCREENING
MUTATIONS
ORGANIC COMPOUNDS
PLANTS
SALMONELLA
SALMONELLA TYPHIMURIUM
SCREENING