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Role of sialic acid in synaptosomal transport of amino acid transmitters

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
;
Active, high-affinity, sodium-dependent uptake of (/sup 14/C)-aminobutyric acid and of the acidic amino acid D-(/sup 3/H)-aspartate was inhibited by pretreatment of synaptosomes with neuraminidase from Vibrio cholerae. Inhibition was of a noncompetitive type and was related to the amount of sialic acid released. The maximum accumulation ratios of both amino acids (intracellular (amino acid)/extracellular (amino acid)) remained largely unaltered. Treatment with neuraminidase affected neither the synaptosomal energy levels nor the concentration of internal potassium. It is suggested that the ..gamma..-aminobutyric acid and acidic amino acid transporters are glycosylated and that sialic acid is involved in the operation of the carrier proteins directly and not through modification of driving forces responsible for amino acid uptake.
Research Organization:
Univ. of Pennsylvania, Philadelphia
OSTI ID:
6077196
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:6; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMIDASES
AMINO ACIDS
AMINOBUTYRIC ACID
ANIMAL CELLS
ANIMALS
ASPARTIC ACID
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
BIOELECTRICITY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHEMISTRY
DRUGS
ELECTRICITY
ENZYMES
HYDROLASES
KINETICS
LABELLED COMPOUNDS
LEUCINE
MAMMALS
MEMBRANE TRANSPORT
MONOSACCHARIDES
NERVE CELLS
NEUROREGULATORS
NON-PEPTIDE C-N HYDROLASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
RATS
REACTION KINETICS
RODENTS
SACCHARIDES
SIALIC ACID
SOMATIC CELLS
TRITIUM COMPOUNDS
VERTEBRATES