Restriction fragment length polymorphism of the HLA-DP subregion and correlations to HLA-DP phenotypes

Journal Article · Sun Mar 01 04:00:00 EST 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.6.1644· OSTI ID:6076848

Hyldig-Nielsen, J J; Morling, N; Oedum, N; Ryder, L P; Platz, P; Jakobsen, B; Svejgaard, A

The restriction fragment length polymorphism (RFLP) of the class II HLA-DP subregion of the major histocompatibility complex (MHC) of humans has been unraveled by Southern blotting using DP/sub ..cap alpha../ and DP/sub ..beta../ probes in a study of 46 unrelated individuals with known HLA-DP types. Contrary to earlier preliminary findings with a limited number of enzymes, the RFLP appears to be quite extensive both with the DP/sub ..beta../ (14 different DNA markers defined by individual fragments or clusters thereof) and the DP/sub ..cap alpha../ (8 markers) probes, especially when enzyme recognizing only four base pairs were used. A few markers were absolutely or strongly associated with individual DP antigens, whereas most were associated with two or more DP antigens as defined by primed lymphocyte typing. Thus, Southern blotting seems feasible for typing for most DP determinants by specific fragments or subtraction between the various more broadly reactive DNA markers, and the RFLP provides further information on the DP subregion in addition to that provided by primed lymphocyte typing. In two recombinant families, the DP/sub ..beta../ and DP/sub ..cap alpha../ DNA markers segregated with DP antigens, whereas the DR/sub ..beta../, DQ/sub ..beta../, DQ/sub ..cap alpha../, and DX/sub ..cap alpha../ markers followed the DR and DQ antigens.

• Research Organization: State Univ. Hospital (Rigshospitalet), Copenhagen, Denmark
• OSTI ID: 6076848
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:6; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BIOLOGICAL EVOLUTION
BIOLOGICAL MATERIALS
BIOLOGY
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DNA
GENES
GENETICS
GLOBULINS
IMMUNOGLOBULINS
IMMUNOLOGY
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RECOMBINANT DNA
SOMATIC CELLS
VERTEBRATES