AraC and AraCDP-L-dipalmitin uptake by mouse leukemia cells

Conference · Fri May 01 04:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6076595

Schliselfeld, L; Hong, C I; Nechaev, A; Buchheit, D

1-..beta..-D-Arabinofuranosylcytosine (araC) is rapidly taken up by araC-sensitive mouse leukemia cells L1210/0 and P388/0 at 2.17 and 3.78 pmoles/min/10/sup 6/ viable cells, respectively. These sensitive cell-lines also had dC-kinase of 10-98 pmoles/min/mg soluble protein. After 1 hr of araC uptake by L1210/0 less then 5% was AraC and about 80% was araCTP. In contrast P388/0 contained 17% and 38% as araC, and 5.1% and 59% as araCTP, respectively. The araC-resistant cell-lines L1210/araC/(A) and P388/araC took up very little AraC, 0.10-0.13 pmoles/min/10/sup 6/ viable cells, had negligible dC-kinase, and contained 50% or more of drug as araC. Fresh L1210/araC/(B) cells exhibited a week resistance to araC, but they had a high uptake rate, high dC-kinase, and good phosphorylation of nucleoside like the sensitive cells. However, after maintaining these cells in mice for 1 yr the L1210/araC/(B) changed to highly araC-resistant cells that tookup little araC and have negligible dC-kinase. (5-/sup 3/H)Arabinofuranosylcytosine-5'-diphosphate-dipalmitin (araCDP-L-dipalmitin) prevents the growth of the sensitive cells, P388/araC, and L1210/araC/(B); however it has no effect on L1210/araC/(A). All of the araC-sensitive and resistant cell-lines took up the conjugate at a very high rate, 500 to 1500 pmole/10/sup 6/ viable cells during the first minute. Little of the tritium was solubilized by extracting the cells with 0.5 N HCLO/sub 4/, but most of it was extracted from all of the cell-lines by CHCL/sub 3//sup 4//methanol/H/sub 2/O. Analyses of the CHCL/sub 3//methanol/H/sub 2/O extracts of araC-sensitive and araC-resistant cells by TLC will be discussed.

• Research Organization: Roswell Park Memorial Institute, Buffalo, NY
• OSTI ID: 6076595
• Report Number(s): CONF-870644-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
CELL PROLIFERATION
CHEMICAL REACTIONS
DISEASES
ENZYME ACTIVITY
ENZYMES
HEMIC DISEASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKEMIA
MAMMALS
METABOLISM
MICE
NEOPLASMS
NUCLEOTIDES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
RODENTS
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
TUMOR CELLS
UPTAKE
VERTEBRATES