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Hepatic metabolism of colloidal gold-low-density lipoprotein complexes in the rat: evidence for bulk excretion of lysosomal contents into bile

Journal Article · · Hepatol.; (United States)
; ;

Rats were treated with 17 alpha-ethinyl estradiol to induce high levels of low-density lipoprotein receptors in hepatocytes. When these rats were given intravenous injections of low-density lipoprotein-colloidal gold complexes, most of the gold (labeled with /sup 195/Au) appeared to be taken up by Kupffer cells, as were complexes of colloidal gold with albumin or polyvinylpyrrolidone. However, when these rats were also administered gadolinium chloride, which blocks Kupffer cell activity, most of the low-density lipoprotein-gold (but not gold complexed with albumin or polyvinylpyrrolidone) was taken up into hepatocytes by receptor-mediated endocytosis and concentrated in peribiliary lysosomes, as determined by electron microscopy. Colloidal gold taken up as a complex with low-density lipoprotein was excreted into the feces via the common bile duct at a maximal rate of about 5% daily, 4 to 12 days after injection. Thereafter, the rate of gold excretion fell off until reaching a plateau after 3 weeks. At this late time, most of the colloidal gold was shown by electron microscopy to be in Kupffer cells, whereas earlier (6 days after injection) it was contained mainly in older hepatocytic lysosomes, identified by lipofuscin granules. It is concluded that, in rats, hepatocytic lysosomes empty most of their contents into bile every week or two, apparently by exocytosis.

Research Organization:
Univ. of California, San Francisco (USA)
OSTI ID:
6075629
Journal Information:
Hepatol.; (United States), Journal Name: Hepatol.; (United States) Vol. 9:3; ISSN HPTLD
Country of Publication:
United States
Language:
English

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Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BILE
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CELL CONSTITUENTS
COLLOIDS
COMPLEXES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISPERSIONS
ELECTRON CAPTURE RADIOISOTOPES
ELECTRON MICROSCOPY
ESTRADIOL
ESTRANES
ESTROGENS
GLANDS
GOLD 195
GOLD ISOTOPES
HEAVY NUCLEI
HORMONES
HYDROXY COMPOUNDS
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIPIDS
LIPOPROTEINS
LIVER
LYSOSOMES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
METABOLISM
MICROSCOPY
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PROTEINS
RADIOISOTOPES
RATS
RECEPTORS
RODENTS
SECONDS LIVING RADIOISOTOPES
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
VERTEBRATES