Lysosomal and endosomal heterogeneity in the liver: A comparison of the intracellular pathways of endocytosis in rat liver cells

Journal Article · Thu Jan 31 23:00:00 EST 1991 · Hepatology (Baltimore); (USA)

DOI:https://doi.org/10.1002/hep.1840130209· OSTI ID:6010166

Kindberg, G M; Tolleshaug, H; Gjoen, T; Berg, T ^[1]

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1. Univ. of Oslo (Norway)

Air-filled albumin microspheres, asialoorosomucoid and formaldehyde-treated serum albumin are selectively taken up by endocytosis in rat liver Kupffer cells, parenchymal cells and endothelial cells, respectively. Intracellular transport and degradation of endocytosed material were studied by subcellular fractionation in sucrose and Nycodenz gradients after intravenous injection of the ligand. By using ligands labeled with 125I-tyramine-cellobiose, the subcellular distribution of labeled degradation products can be studied because they are trapped at the site of formation. The results show that the kinetics of intracellular transport are different in hepatic parenchymal, endothelial and Kupffer cells. In endothelial cells, the ligand is associated with two types of endosomes during the first minutes after internalization and then is transferred rapidly to the lysosomes. In parenchymal cells, 125I-tyramine-cellobiose-asialoorosomucoid was located in a relatively slowly sedimenting vesicle during the first minute after internalization and subsequently in denser endosomes. Degradation of 125I-tyramine-cellobiose-asialoorosomucoid in parenchymal cells started later than that of 125I-tyramine-cellobiose-formaldehyde-treated serum albumin in endothelial cells. Furthermore, the ligand seemed to be transferred relatively slowly from endosomes to lysosomes, and most of the undegraded ligand was in the endosomes. The rate-limiting step of proteolysis in parenchymal cells is probably the transport from endosomes to lysosomes. In Kupffer cells, most 125I-tyramine-cellobiose-microspheres are found as undegraded material in very dense endosomes up to 3 hr after injection. After 20 hr, most of the ligand is degraded in lysosomes distributed at a lower density than the endosomes in Nycodenz and sucrose gradients.

• OSTI ID: 6010166
• Journal Information: Hepatology (Baltimore); (USA), Journal Name: Hepatology (Baltimore); (USA) Vol. 13:2; ISSN HPTLD; ISSN 0270-9139
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
AMINES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BIOLOGICAL PATHWAYS
BODY
CARBOHYDRATES
CELL CONSTITUENTS
CELLOBIOSE
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISACCHARIDES
DISTRIBUTION
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
GLANDS
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGANDS
LIVER
LIVER CELLS
LYSOSOMES
MAMMALS
MEMBRANE TRANSPORT
METABOLISM
MICROSPHERES
NUCLEI
ODD-EVEN NUCLEI
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PHAGOCYTOSIS
PHENOLS
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SACCHARIDES
SOMATIC CELLS
SUBCELLULAR DISTRIBUTION
SYMPATHOMIMETICS
TISSUES
TRACER TECHNIQUES
TYRAMINE
VERTEBRATES