Maximizing gene expression
Book
·
OSTI ID:6074227
The cornerstone of the new biotechnology industry is the ability to construct organisms which, when cultured, will produce large quantities of desired proteins. Each chapter of Maximising Gene Expression discusses a particular question related to the overall series of events which leads to obtaining large amounts of protein. Topics include the control of plasmid replication and stable inheritance in E. Coli and yeast; the initiation of mRNA synthesis; the determinants of mRNA stability; the control of the translation initiation process; what is known about protein degradation. Contents: E. Coli promoters. Yeast promoters. Protein coding genes of higher eukarayotes. The instability of messenger RNA in bacteria. Replication control of the ColE1-type plasmids. Copy number and stability of yeast plasmids. Translational initiation. Biased codon usage. The selective degradation of abnormal proteins in bacteria. Detection of proteins produced by recombinant DNA techniques. Mechanism and practice.
- OSTI ID:
- 6074227
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200 -- Biochemistry
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOSYNTHESIS
BIOTECHNOLOGY
CELL CONSTITUENTS
CHEMICAL REACTIONS
DECOMPOSITION
DNA
ESCHERICHIA COLI
FUNGI
GENE AMPLIFICATION
GENE REGULATION
GENE REPRESSORS
GENES
GENETIC ENGINEERING
MESSENGER-RNA
MICROORGANISMS
NUCLEIC ACIDS
NUCLEOPROTEINS
OPTIMIZATION
ORGANIC COMPOUNDS
PLANNING
PLANTS
PLASMIDS
PROTEINS
RECOMBINANT DNA
RNA
SYNTHESIS
TRANSCRIPTION
YEASTS
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOSYNTHESIS
BIOTECHNOLOGY
CELL CONSTITUENTS
CHEMICAL REACTIONS
DECOMPOSITION
DNA
ESCHERICHIA COLI
FUNGI
GENE AMPLIFICATION
GENE REGULATION
GENE REPRESSORS
GENES
GENETIC ENGINEERING
MESSENGER-RNA
MICROORGANISMS
NUCLEIC ACIDS
NUCLEOPROTEINS
OPTIMIZATION
ORGANIC COMPOUNDS
PLANNING
PLANTS
PLASMIDS
PROTEINS
RECOMBINANT DNA
RNA
SYNTHESIS
TRANSCRIPTION
YEASTS