Vector for high level gene expression
Patent
·
OSTI ID:5998302
A method is described for expressing a gene encoding a desired protein in an E. coli host at elevated levels of expression, which comprises: (a) inserting a structural gene encoding a desired protein and the trpt sequence of the E. coli tryptophan operon into a vector replicable in an E. coli host, such that the sequence is adjacent to and downstream from the translation termination codon of the structural gene; (b) transforming the E. coli host with the vector; and (c) expressing the structural gene in the thus transformed E. coli host.
- Assignee:
- Genex Corp., Gaithersburg, MD
- Patent Number(s):
- US 4798791
- OSTI ID:
- 5998302
- Country of Publication:
- United States
- Language:
- English
Similar Records
Maximizing gene expression
Cloning, expression and stable maintenance of a bacteriophage DNA repair gene in E. coli
Combined protein construct and synthetic gene engineering for heterologous protein expression and crystallization using Gene Composer
Book
·
Tue Dec 31 23:00:00 EST 1985
·
OSTI ID:6074227
Cloning, expression and stable maintenance of a bacteriophage DNA repair gene in E. coli
Conference
·
Thu May 01 00:00:00 EDT 1986
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:6905843
Combined protein construct and synthetic gene engineering for heterologous protein expression and crystallization using Gene Composer
Journal Article
·
Mon Nov 30 23:00:00 EST 2009
· BMC Biotechnology
·
OSTI ID:1005813