Isolation and characterization of the inositol trisphosphate receptor from smooth muscle

Journal Article · Wed Feb 28 23:00:00 EST 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.6.2132· OSTI ID:6073682

Chadwick, C C; Saito, A; Fleischer, S ^[1]

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1. Vanderbilt Univ., Nashville, TN (USA)

The release of Ca{sup 2+} from internal stores is requisite to muscle contraction. In skeletal muscle and heart, the Ca{sup 2+} release channels (ryanodine receptor) of sarcoplasmic reticulum, involved in excitation-contraction coupling, have recently been isolated and characterized. In smooth muscle, inositol 1,4,5-trisphosphate (IP{sub 3}) is believed to mobilize Ca{sup 2+} from internal stores and thereby modulate contraction. The authors describe the isolation of an IP{sub 3} receptor from smooth muscle. Bovine aorta smooth muscle microsomes were solubilized with 3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate, and the IP{sub 3} receptor was purified by sucrose gradient centrifugation and column chromatography with heparin-agarose and wheat germ agglutinin-agarose. The receptor is an oligomer of a single polypeptide with a M{sub r} of 224,000 as determined by SDS/PAGE. Negative-staining electron microscopy reveals that the receptor is a large pinwheel-like structure having surface dimensions of {approx}250 {times} 250 {angstrom} with fourfold symmetry. The IP{sub 3} receptor from smooth muscle is similar to the ryanodine receptor with regard to its large size and fourfold symmetry, albeit distinct with regard to appearance, protomer size, and ligand binding.

• OSTI ID: 6073682
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 87:6; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMICAL REACTION KINETICS
CARBOHYDRATES
CELL CONSTITUENTS
ELECTRON MICROSCOPY
ELECTROPHORESIS
ENDOPLASMIC RETICULUM
HYDROGEN COMPOUNDS
INOSITOLS
ISOTOPE APPLICATIONS
KINETICS
MEMBRANE PROTEINS
MICROSCOPY
MONOSACCHARIDES
MUSCLES
ORGANIC COMPOUNDS
ORGANOIDS
PROTEINS
PURIFICATION
RADIORECEPTOR ASSAY
REACTION KINETICS
RECEPTORS
SACCHARIDES
SARCOPLASMIC RETICULUM
TRACER TECHNIQUES
TRANSMISSION ELECTRON MICROSCOPY
TRITIUM COMPOUNDS