Malate synthase a membrane protein
Conference
·
· Plant Physiol., Suppl.; (United States)
OSTI ID:6073154
Malate synthase (MS) is generally regarded as a peripheral membrane protein, and believed by some to be ontogenetically associated with ER. However, immuno- and cyto-chemical in situ localizations show MS throughout the matrix of cotton (and cucumber) glyoxysomes, not specifically near their boundary membranes, nor in ER. Only a maximum of 50% MS can be solubilized from cotton glyoxysomes with 1% Triton X-100, 2mM Zwittergen 14, or 10mM DOC +/- salts. Cotton MS does not incorporate /sup 3/H-glucosamine in vivo, nor does it react with Con A on columns or blots. Cotton MS banded with ER in sucrose gradients (20-40%) in Tricine after 3h, but not after 22h in Tricine or Hepes, or after 3h in Hepes or K-phosphate. Collectively the authors data are inconsistent with physiologically meaningful MS-membrane associations in ER or glyoxysomes. It appears that experimentally-induced aggregates of MS migrate in ER gradients and occur in isolated glyoxysomes. These data indicate that ER is not involved in synthesis or modification of cottonseed MS prior to its import into the glyoxysomal matrix.
- Research Organization:
- Arizona State Univ., Tempe
- OSTI ID:
- 6073154
- Report Number(s):
- CONF-8707108-
- Conference Information:
- Journal Name: Plant Physiol., Suppl.; (United States) Journal Volume: 83:4
- Country of Publication:
- United States
- Language:
- English
Similar Records
Phosphorylation of glyoxysomal malate synthase from castor oil seed endosperm and cucumber cotyledon
In vivo phospholipid biosynthesis in cotton cotyledons during glyoxysome enlargement
Relationship between 5S and 20S forms of malate synthase in maturing cottonseeds
Journal Article
·
Fri Mar 31 23:00:00 EST 1989
· Plant Physiology, Supplement; (USA)
·
OSTI ID:6611070
In vivo phospholipid biosynthesis in cotton cotyledons during glyoxysome enlargement
Conference
·
Tue May 01 00:00:00 EDT 1990
· Plant Physiology, Supplement; (USA)
·
OSTI ID:5850895
Relationship between 5S and 20S forms of malate synthase in maturing cottonseeds
Conference
·
Tue Mar 31 23:00:00 EST 1987
· Plant Physiol., Suppl.; (United States)
·
OSTI ID:5903871
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADDITIVES
AMINES
BIOLOGICAL FUNCTIONS
BIOSYNTHESIS
CARBOHYDRATES
CELL CONSTITUENTS
CELL MEMBRANES
CENTRIFUGATION
COTTON PLANTS
DETERGENTS
DISTRIBUTION
EMULSIFIERS
ENDOPLASMIC RETICULUM
ENZYMES
FUNCTIONS
GLUCOSAMINE
HEXOSAMINES
HEXOSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIGASES
MEMBRANES
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANOIDS
PLANT CELLS
PLANTS
PROTEINS
SACCHARIDES
SEDIMENTATION
SEPARATION PROCESSES
SUBCELLULAR DISTRIBUTION
SURFACTANTS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
WETTING AGENTS
59 BASIC BIOLOGICAL SCIENCES
ADDITIVES
AMINES
BIOLOGICAL FUNCTIONS
BIOSYNTHESIS
CARBOHYDRATES
CELL CONSTITUENTS
CELL MEMBRANES
CENTRIFUGATION
COTTON PLANTS
DETERGENTS
DISTRIBUTION
EMULSIFIERS
ENDOPLASMIC RETICULUM
ENZYMES
FUNCTIONS
GLUCOSAMINE
HEXOSAMINES
HEXOSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIGASES
MEMBRANES
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANOIDS
PLANT CELLS
PLANTS
PROTEINS
SACCHARIDES
SEDIMENTATION
SEPARATION PROCESSES
SUBCELLULAR DISTRIBUTION
SURFACTANTS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
WETTING AGENTS