Relationship between 5S and 20S forms of malate synthase in maturing cottonseeds
Conference
·
· Plant Physiol., Suppl.; (United States)
OSTI ID:5903871
Malate synthase (MS) activity appears and increases during seed maturation persists during desiccation, then increases again following germination. Because different modes of synthesis and organelle import of MS may occur in maturing and germinated seeds, a comparative study was performed. A comparison of immunoprecipitations from in-vivo-labeled seeds (/sup 35/S-met) and in-vitro translations of Poly A+ RNA (wheat germ) showed no detectable differences in subunit mol wt. When MS activity first appears (42 DPA) only the cytosolic 5S form is found in rate-zonal gradients (5-25% sucrose). At 48 DPA, O d, and 48 h germinated seeds both the 5S and glyoxysomal 20S forms are present, with the 20S becoming more prevalent. Western blots of SDS-PAGE gels showed that no other form(s) of MS (inactive) are present in rate-zonal fractions. Calculations of radiospecific activity (per MS activity) of 5S and 20S forms radiolabeled in vivo (/sup 35/S-met) at various time periods provided further convincing evidence that there is a 5S precursor to 20S product relationship during both seed maturation and seedling growth.
- Research Organization:
- Arizona State Univ., Tempe
- OSTI ID:
- 5903871
- Report Number(s):
- CONF-8707108-
- Conference Information:
- Journal Name: Plant Physiol., Suppl.; (United States) Journal Volume: 83:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CENTRIFUGATION
CEREALS
COTTON PLANTS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTROPHORESIS
ENZYME ACTIVITY
ENZYMES
EVEN-ODD NUCLEI
GERMINATION
GRASS
GROWTH
HYBRIDIZATION
IMMUNOASSAY
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGASES
LIGHT NUCLEI
LIPOTROPIC FACTORS
METHIONINE
MOLECULAR WEIGHT
NUCLEI
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANOIDS
PLANT GROWTH
PLANTS
PRECURSOR
RADIOISOTOPES
RNA
SEDIMENTATION
SEEDLINGS
SEEDS
SEPARATION PROCESSES
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
TRANSLOCATION
WHEAT
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CENTRIFUGATION
CEREALS
COTTON PLANTS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTROPHORESIS
ENZYME ACTIVITY
ENZYMES
EVEN-ODD NUCLEI
GERMINATION
GRASS
GROWTH
HYBRIDIZATION
IMMUNOASSAY
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGASES
LIGHT NUCLEI
LIPOTROPIC FACTORS
METHIONINE
MOLECULAR WEIGHT
NUCLEI
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANOIDS
PLANT GROWTH
PLANTS
PRECURSOR
RADIOISOTOPES
RNA
SEDIMENTATION
SEEDLINGS
SEEDS
SEPARATION PROCESSES
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
TRANSLOCATION
WHEAT