Role of glutathione peroxidase in the radiation response of mouse kidney

Journal Article · Mon May 01 00:00:00 EDT 1989 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

DOI:https://doi.org/10.1016/0360-3016(89)90286-1· OSTI ID:6073038

Stevens, G N; Joiner, M C; Joiner, B; Johns, H; Stratford, M R

Glutathione peroxidase (GSH-Px) has been implicated in mediating the radioprotective effects of glutathione (GSH). This hypothesis was tested in vivo by determining the effect of GSH-Px depletion on the radiation response of murine kidneys. Renal GSH-Px levels were depleted to 17% of control values by feeding animals a selenium deficient diet for 6 weeks; this had no significant effect on renal levels of GSH or GSH-S-transferase (GST). However, we also tested the effect of direct depletion of GSH to 3-4% of control values, using a combination of DL-buthionine sulphoximine (BSO) and diethyl maleate (DEM). Kidneys with normal or depleted levels of GSH-Px and/or GSH were irradiated with 240kVp X rays (2 fractions, 7 days apart to minimize intestinal injury). Mice breathed 7% oxygen during irradiation. Renal damage was assessed at 20, 25, and 32 weeks after the first fraction of X rays, in terms of reduced hematocrit and renal clearance of /sup 51/Cr-EDTA. Depletion of GSH-Px levels to 17% of control did not alter renal radiosensitivity, but depletion of GSH to 3-4% of control values radiosensitized the kidney by a factor of 1.4. Depletion of both GSH and GSH-Px together did not radiosensitize the kidney any more than was achieved by GSH depletion alone.

• Research Organization: Gray Laboratory of the Cancer Research Campaign, Middlesex (England)
• OSTI ID: 6073038
• Journal Information: Int. J. Radiat. Oncol., Biol. Phys.; (United States), Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Vol. 16:5; ISSN IOBPD
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560152* -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CHELATING AGENTS
CHROMIUM 51
CHROMIUM ISOTOPES
DIET
EDTA
ELECTROMAGNETIC RADIATION
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
EVEN-ODD NUCLEI
FRACTIONATED IRRADIATION
INTERMEDIATE MASS NUCLEI
IONIZING RADIATIONS
IRRADIATION
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
MAMMALS
MICE
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEROXIDASES
RADIATIONS
RADIOISOTOPES
RADIOSENSITIVITY
RADIOSENSITIVITY EFFECTS
RODENTS
TRACER TECHNIQUES
VERTEBRATES
X RADIATION