Characterization of embryo-specific genes
The objective of the proposed research is to characterize the structure and function of a set of genes whose expression is regulated in embryo development, and that is not expressed in mature tissues -- the embryonic genes. In the last two years, using cDNA clones, we have isolated 22 cDNA clones, and characterized the expression pattern of their corresponding RNA. At least 4 cDNA clones detect RNAs of embryonic genes. These cDNA clones detect RNAs expressed in somatic as well as zygotic embryos of carrot. Using the cDNA clones, we screened the genomic library of carrot embryo DNA, and isolated genomic clones for three genes. The structure and function of two genes DC 8 and DC 59 have been characterized and are reported in this paper.
- Research Organization:
- California Univ., Berkeley, CA (USA). Dept. of Physics
- Sponsoring Organization:
- DOE/ER
- DOE Contract Number:
- FG03-87ER13698
- OSTI ID:
- 6072092
- Report Number(s):
- DOE/ER/13698-T2; ON: DE91006869
- Country of Publication:
- United States
- Language:
- English
Similar Records
Characterization of embryo-specific genes
Characterization of embryo-specific genes. Final report, April 1, 1987--March 31, 1992
Related Subjects
CARROTS
DNA-CLONING
EMBRYONIC CELLS
GENE REGULATION
DNA SEQUENCING
EMBRYOS
PROGRESS REPORT
PROTEINS
RNA
ANIMAL CELLS
CLONING
DNA HYBRIDIZATION
DOCUMENT TYPES
FOOD
HYBRIDIZATION
MAGNOLIOPHYTA
MAGNOLIOPSIDA
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLANTS
STRUCTURAL CHEMICAL ANALYSIS
VEGETABLES
550400* - Genetics
550200 - Biochemistry