Effect of /sup 67/Cu and /sup 99/Mo labeled tetrathiomolybdate on the distribution of /sup 67/Cu, Cu, and /sup 99/Mo in bile fractions in sheep

Journal Article · Sat Apr 01 04:00:00 EST 1989 · J. Inorg. Biochem.; (United States)

DOI:https://doi.org/10.1016/0162-0134(89)84014-0· OSTI ID:6071878

Gooneratne, R; Laarveld, B; Christensen, D

The effect of intravenous administration of /sup 67/Cu and /sup 99/Mo labeled tetrathiomolybdate (TTM) on the appearance of /sup 67/Cu, stable Cu, and /sup 99/Mo in gel chromatographic fractions of bile was examined in sheep fed either 5 or 35 mg Cu kg-1 DM. Peak excretory periods of biliary /sup 67/Cu, stable Cu, and /sup 99/Mo were observed at 30 min-1.25 hr, 2-3 hr, and 11-13 hr after /sup 67/Cu and after /sup 99/Mo labeled TTM. Sephadex G-75 gel filtration of bile samples collected at 1, 3, and 12 hr after /sup 67/Cu administration revealed two major protein peaks of molecular weights of greater than 80,000 (peak I) and 7,000 (peak II) containing both /sup 67/Cu and Cu. But the ratio of /sup 67/Cu in the two peaks varied with time of bile collection. The ratio of areas of peak I:II 1 hr after /sup 67/Cu administration was approximately 0.48; at 3 hr, 0.62, and at 12 hr 1.35. Tetrathiomolybdate administration increased both /sup 67/Cu and stable Cu in bile by severalfold and induced a major shift of Cu into the higher molecular weight protein fraction. The experiments confirm the effectiveness of TTM as a ''decoppering'' agent. Furthermore, TTM not only promoted bile Cu excretion, but it also increased the incorporation of Cu into the macromolecular fraction. This may limit enterohepatic circulation of biliary Cu and thereby cause an overall Cu depletion and a negative Cu balance.

• Research Organization: Lincoln College, Canterbury (New Zealand)
• OSTI ID: 6071878
• Journal Information: J. Inorg. Biochem.; (United States), Journal Name: J. Inorg. Biochem.; (United States) Vol. 35:4; ISSN JIBID
• Country of Publication: United States
• Language: English

Related Subjects

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560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BILE
BIOLOGICAL MATERIALS
BODY FLUIDS
CHROMATOGRAPHY
COPPER
COPPER 67
COPPER ISOTOPES
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ELEMENTS
EVEN-ODD NUCLEI
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
INTRAVENOUS INJECTION
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
ISOTOPES
MAMMALS
MATERIALS
METABOLISM
METALS
MOLECULAR WEIGHT
MOLYBDENUM
MOLYBDENUM 99
MOLYBDENUM ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
RADIOISOTOPES
RUMINANTS
SEPARATION PROCESSES
SHEEP
TRACER TECHNIQUES
TRANSITION ELEMENTS
VERTEBRATES