Anemia of the Belgrade rat: evidence for defective membrane transport of iron

Journal Article · Wed Jul 01 04:00:00 EDT 1987 · Blood; (United States)

OSTI ID:6068883

Bowen, B J; Morgan, E H

The mechanisms underlying the impaired utilization of transferrin-bound iron by erythroid cells in the anemia of the Belgrade laboratory rat were investigated using reticulocytes from homozygous anemic animals and transferrin labeled with /sup 59/Fe and /sup 125/I. The results were compared with those obtained using reticulocytes from phenylhydrazine-treated rats and iron-deficient rats. Each step in the iron uptake mechanism was investigated, ie, transferrin-receptor interaction, transferrin endocytosis, iron release from transferrin, and transferrin exocytosis. Although there were quantitative differences, no fundamental difference was found in any of the abovementioned aspects of cellular function when the reticulocytes from Belgrade rats were compared with those from iron-deficient animals. The basic defect in the Belgrade reticulocytes must therefore reside in subsequent steps in iron uptake, after it is released from transferrin within endocytotic vesicles, ie, in the mechanism by which it is transferred across the lining membrane of the vesicles into the cell cytosol. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of reticulocyte ghosts extracts demonstrated a prominent protein band of mol wt 69,000 that was absent or present only in low concentration extracts from the other two types of reticulocytes. This may be a result of the genetic defect.

• Research Organization: Univ. of Western Australia, Nedlands
• OSTI ID: 6068883
• Journal Information: Blood; (United States), Journal Name: Blood; (United States) Vol. 70:1; ISSN BLOOA
• Country of Publication: United States
• Language: English

Related Subjects

550901 -- Pathology-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANEMIAS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CELL MEMBRANES
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ELEMENTS
ERYTHROCYTES
EVEN-ODD NUCLEI
GLOBULINS
GLOBULINS-BETA
HEMIC DISEASES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
IRON
IRON 59
IRON ISOTOPES
ISOTOPES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MEMBRANE TRANSPORT
MEMBRANES
METALLOPROTEINS
METALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PATHOGENESIS
PROTEINS
RADIOISOTOPES
RATS
RECEPTORS
RETICULOCYTES
RODENTS
SYMPTOMS
TRANSFERRIN
TRANSITION ELEMENTS
VERTEBRATES