Erythroblast transferrin receptors and transferrin kinetics in iron deficiency and various anemias

Journal Article · Mon Jun 01 04:00:00 EDT 1987 · Am. J. Hematol.; (United States)

DOI:https://doi.org/10.1002/ajh.2830250205· OSTI ID:6274830

Muta, K; Nishimura, J; Ideguchi, H; Umemura, T; Ibayashi, H

To clarify the role of transferrin receptors in cases of altered iron metabolism in clinical pathological conditions, we studied: number of binding sites; affinity; and recycling kinetics of transferrin receptors on human erythroblasts. Since transferrin receptors are mainly present on erythroblasts, the number of surface transferrin receptors was determined by assay of binding of /sup 125/I-transferrin and the percentage of erythroblasts in bone marrow mononuclear cells. The number of binding sites on erythroblasts from patients with an iron deficiency anemia was significantly greater than in normal subjects. Among those with an aplastic anemia, hemolytic anemia, myelodysplastic syndrome, and polycythemia vera compared to normal subjects, there were no considerable differences in the numbers of binding sites. The dissociation constants (Kd) were measured using Scatchard analysis. The apparent Kd was unchanged (about 10 nmol/L) in patients and normal subjects. The kinetics of endocytosis and exocytosis of /sup 125/I-transferrin, examined by acid treatment, revealed no variations in recycling kinetics among the patients and normal subjects. These data suggest that iron uptake is regulated by modulation of the number of surface transferrin receptors, thereby reflecting the iron demand of the erythroblast.

• Research Organization: Kyushu Univ., Fukuoka, Japan
• OSTI ID: 6274830
• Journal Information: Am. J. Hematol.; (United States), Journal Name: Am. J. Hematol.; (United States) Vol. 25:2; ISSN AJHED
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANEMIAS
ANIMAL CELLS
ANIMAL TISSUES
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
GLOBULINS
GLOBULINS-BETA
HEMATOPOIETIC SYSTEM
HEME
HEMIC DISEASES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
IRON
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LEUKOCYTES
LYMPHOCYTES
MATERIALS
METALLOPROTEINS
METALS
NUCLEI
NUTRITIONAL DEFICIENCY
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOGENESIS
PIGMENTS
PORPHYRINS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SOMATIC CELLS
SYMPTOMS
TISSUES
TRACER TECHNIQUES
TRANSFERRIN
TRANSITION ELEMENTS
UPTAKE