In vivo ESR measurement of free radicals in whole mice

Journal Article · Thu Nov 15 04:00:00 EST 1990 · Biochemical and Biophysical Research Communications; (USA)

DOI:https://doi.org/10.1016/0006-291X(90)91597-L· OSTI ID:6065526

Utsumi, H; Muto, E; Masuda, S; Hamada, A ^[1]

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1. Showa Univ., Tokyo (Japan)

The in vivo measurement of nitroxide radicals in whole mouse was carried out by L-band ESR spectroscopy. Spectra were successively observed in hepatic and bladder domains of female mice after intravenous administration of spin-labeled compounds (CPROXYL or TEMPOL). The signal intensities from both domains decreased gradually. The kinetic constants of clearance in the hepatic domain were 0.09/min for CPROXYL and 0.71/min for TEMPOL. The clearance constants in the bladder domain coincided with those in the hepatic domain within experimental error, whereas the constants in collected blood were 1/7-1/10 of those in the hepatic or bladder domains. The mechanism of clearance of nitroxide radicals in whole mice is discussed.

• OSTI ID: 6065526
• Journal Information: Biochemical and Biophysical Research Communications; (USA), Journal Name: Biochemical and Biophysical Research Communications; (USA) Vol. 172:3; ISSN BBRCA; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BLADDER
BODY
CHALCOGENIDES
CLEARANCE
DIGESTIVE SYSTEM
ELECTRON SPIN RESONANCE
GLANDS
IN VIVO
LIVER
MAGNETIC RESONANCE
MAMMALS
METABOLISM
MICE
NITROGEN COMPOUNDS
NITROGEN OXIDES
ORGANS
OXIDES
OXYGEN COMPOUNDS
RADICALS
RESONANCE
RODENTS
URINARY TRACT
VERTEBRATES