ESR measurement of radical clearance in lung of whole mouse

Journal Article · Fri Jun 14 04:00:00 EDT 1991 · Biochemical and Biophysical Research Communications; (United States)

DOI:https://doi.org/10.1016/0006-291X(91)91871-9· OSTI ID:5448065

Takeshita, K; Utsumi, H; Hamada, A ^[1]

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1. Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo (Japan)

Clearance of the nitroxide radicals, hydroxy-TEMPO and carboxy-PROxYL, in whole-mouse lung was directly measured by in vivo ESR. After injecting a nitroxide radical, distribution of the nitroxide radical all over the lung was confirmed by ESR imaging. The ESR signal of hydroxy-TEMPO was reduced in the lung and the clearance obeyed first-order kinetics, whereas the signal of carboxy-PROxYL remained constant. Comparison of the clearance rates of live and dead mice indicated the presence of 2 different clearance systems in the lung: loss of its paramagnetism in the lung, and transfer from alveolar to the blood circulation system.

• OSTI ID: 5448065
• Journal Information: Biochemical and Biophysical Research Communications; (United States), Journal Name: Biochemical and Biophysical Research Communications; (United States) Vol. 177:2; ISSN BBRCA; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
CHALCOGENIDES
CLEARANCE
ELECTRON SPIN RESONANCE
EXCRETION
LUNG CLEARANCE
MAGNETIC RESONANCE
MAMMALS
METABOLISM
MICE
MUSCLES
NITROGEN COMPOUNDS
NITROGEN OXIDES
OXIDES
OXYGEN COMPOUNDS
RADICALS
RESONANCE
RODENTS
VERTEBRATES