Lithium effects on solute retrieval by excised leaf discs. [Ipomoea tricolor]

Conference · Tue Mar 31 23:00:00 EST 1987 · Plant Physiol., Suppl.; (United States)

OSTI ID:6065272

Madore, M

Excision of Ipomoea tricolor leaf discs results in a transient reduction in membrane transport capability which shows recovery after a 3 h aging period. After the recovery period, aged discs show both an enhanced capacity for uptake of exogenously supplied solutes: ((/sup 14/C)sucrose, (/sup 14/C)glucose, (/sup 14/C)glycine) as well as a reduced loss of photosynthetically derived /sup 14/C-assimilates, suggesting that general solute retrieval mechanisms operating in leaf tissues are initially somehow inhibited by the excision process. Inclusion of lithium (Li/sup +/), a known inhibitor of phosphatidylinositol(PI)-mediated signaling mechanisms involved in the control of cellular Ca/sup 2 +/ homeostasis, in the aging medium completely eliminates the recovery of these retrieval processes. On the other hand, treatment of Li/sup +/-treated discs with myo-inositol, which overcomes Li/sup +/-induced inhibition of the PI signalling system, results in full or nearly complete recovery of solute transport following aging. These data implicate the PI signalling system in the wounding phenomenon that results in the initial loss of solute retrieval capacity in freshly excised discs. It is possible that this control is exerted through control of H/sup +/-ATPase activity which drives transport.

• Research Organization: Univ. of California, Riverside
• OSTI ID: 6065272
• Report Number(s): CONF-8707108-
• Journal Information: Plant Physiol., Suppl.; (United States), Journal Name: Plant Physiol., Suppl.; (United States) Vol. 83:4; ISSN PPYSA
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACID ANHYDRASES
ALDEHYDES
ALKALI METALS
ALKALINE EARTH METALS
AMINO ACIDS
ATP-ASE
BIOLOGICAL EFFECTS
CALCIUM
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CATIONS
CHARGED PARTICLES
CHEMICAL REACTIONS
DISACCHARIDES
ELEMENTS
ENZYMES
ESTERS
FOLIAR UPTAKE
GLUCOSE
GLYCINE
HEXOSES
HYDROLASES
INHIBITION
INJURIES
INOSITOLS
IONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEAVES
LIPIDS
LITHIUM
MEMBRANE TRANSPORT
METALS
MONOSACCHARIDES
OLIGOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORNAMENTAL PLANTS
PHOSPHOHYDROLASES
PHOSPHOLIPIDS
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PLANTS
SACCHARIDES
SACCHAROSE
SOLUTES
SYNTHESIS
TRACER TECHNIQUES
UPTAKE
WOUNDS