Effect of 1-aminocyclopropane-1-carboxylic acid on the production of ethylene in senescing flowers of Ipomoea tricolor Cav

Journal Article · Wed Oct 01 00:00:00 EDT 1980 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.66.4.566· OSTI ID:7099007

Konze, J R; Jones, J F; Boller, T; Kende, H

Application of 1-aminocyclopropane-1-carboxylic acid (ACC) to rib segments excised from flowers of Ipomoea tricolor Cav. resulted in the formation of C/sub 2/H/sub 4/ in greater quantities than produced under natural conditions. The ability of ACC to enhance C/sub 2/H/sub 4/ production was independent of the physiological age of the tissue and its capacity to synthesize C/sub 2/H/sub 4/ without applied ACC. When ACC was fed to rib segments that had been treated with (/sup 14/C)methionine, incorporation of radioactivity into C/sub 2/H/sub 4/ was reduced by 80%. Aminoethoxyvinylglycine and aminooxyacetic acid inhibited C/sub 2/H/sub 4/ production in rib segments of I. tricolor but had no effect on ACC-enhanced C/sub 2/H/sub 4/ production. Protoplasts obtained from flower tissue of I. tricolor did not form C/sub 2/H/sub 4/, even when incubated with methionine or selenomethionine. They produced C/sub 2/H/sub 4/ upon incubation with ACC, however. ACC-dependent C/sub 2/H/sub 4/ production in protoplasts was inhibited by n-propyl gallate, AgCl, CoCl/sub 2/, KCN, Na/sub 2/S, and NaN/sub 3/. ACC-dependent C/sub 2/H/sub 4/ synthesis in rib segments and protoplasts was dependent on O/sub 2/, the K/sub m/ for O/sub 2/ being 1.0 to 1.4% (v/v). These results confirm the following pathway for C/sub 2/H/sub 4/ biosynthesis in I. tricolor: methionine (selenomethionine) ..-->..S-adenosylmethionine (selenoadenosylmethionine) ..-->.. ACC ..-->.. C/sub 2/H/sub 4/.

• Research Organization: Michigan State Univ., East Lansing
• DOE Contract Number: EY-76-C-02-1338
• OSTI ID: 7099007
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 66:4; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETIC ACID
ALKENES
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CARBON 14
CARBON ISOTOPES
CARBOXYLIC ACIDS
CHEMICAL ANALYSIS
ENZYME ACTIVITY
ETHYLENE
EVEN-EVEN NUCLEI
FLOWERS
HYDROCARBONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPOTROPIC FACTORS
METABOLISM
METHIONINE
MONOCARBOXYLIC ACIDS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PLANT TISSUES
PLANTS
QUANTITATIVE CHEMICAL ANALYSIS
QUANTITY RATIO
RADIOISOTOPES
REACTION KINETICS
SENSITIVITY
SUBSTRATES
SYNTHESIS
TIME DEPENDENCE
YEARS LIVING RADIOISOTOPES