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Thermal sensitivity and radiosensitization in V79 cells after BrdUrd or IdUrd incorporation

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3576060· OSTI ID:6064375
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Chinese hamster V79 cells were exposed to 10/sup -5/ moles/liter bromodeoxyuridine (BrdUrd) or iododeoxyuridine (IdUrd) for 16 or 29 hr and then tested for thermal sensistivity, radiosensitivity, and sensitivity to the combined treatments of heat and radiation. BrdUrd and IdUrd treatment of cells resulted in enhanced radiosensitivity which increased with exposure time but had litte or no effect on thermal sensitivity. For 24.0/sup 0/C heating, no effect was observed, while for 45.0/sup 0/C heating, a small decrease in thermal sensitivity occurred for both 16- and 29-hr exposure times. In the combined treatment of heat and radiation, the presence of BrdUrd or IdUrd resulted in about the same thermal enhancement in radiosensitivity. BrdUrd and IdUrd uptake into cellular DNA were measured using high-pressure liquid chromatography and, after a 29-hr exposure to 10/sup -5/ moles/liter of BrdUrd or IdUrd, approximately 40% of the thymidine was substituted. 33 references, 5 figures, 2 tables.

Research Organization:
Atomic Energy of Canada Ltd. Research Co., Pinawa, Manitoba
OSTI ID:
6064375
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 98; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560201 -- Thermal Effects-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
AZINES
BODY
BROMOURACILS
BUDR
CHROMATOGRAPHY
DATA
DNA
DRUGS
EXPERIMENTAL DATA
HAMSTERS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INFORMATION
IODODEOXYURIDINE
IODOURACILS
LIQUID COLUMN CHROMATOGRAPHY
LUNGS
MAMMALS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RADIOSENSITIVITY
RADIOSENSITIVITY EFFECTS
RESPIRATORY SYSTEM
RIBOSIDES
RODENTS
SEPARATION PROCESSES
SYNERGISM
TEMPERATURE EFFECTS
THYMIDINE
UPTAKE
URACILS
VERTEBRATES